MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03260cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
210630s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.11.07.Ph.D.2021.Am.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Amira Mohamed Abbas Salama |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Prevalence of mycobacteria other than tuberculosis in clinical isolates from Cairo university hospital in one year duration and species identification using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry / |
| Statement of responsibility, etc. |
Amira Mohamed Abbas Salama ; Supervised Sahar Mohamed Khairat , Mohamed Ahmed Awad Elbashar, Maha Ali khalaf |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
معدل إنتشار المتفطرات غير السل فى العزلات الإكلينيكية من مستشفى جامعة القاهرة لمدة عام واحد والتعرف على الفصائل باستخدام جهاز قياس الطيف الكتلى بمساعدة الليزر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Amira Mohamed Abbas Salama , |
| Date of publication, distribution, etc. |
2021 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
122 P . : |
| Other physical details |
charts , facsmilies ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
My cobacteria other than tuberculosis (MOTT) are globally recognized as pathogens especially in immune-compromised population. Species-level identification of MOTT is becoming increasingly clinically important. Patients and Methods: A total of 810 samples were involved and the positive culture isolates underwent Identification Test for MOTT identification then species identification by LCD array MycoDirect 1.7 and MALDI-TOF MS. Results: A total of 58 mycobacteria culture positive out of suspected 810 samples which were collected from Cairo University Hospital, Egypt. The prevalence of MOTT was 0.7 % among total samples and 10.3% among positive culture samples MOTT species identification was done by MALDI-TOF MS from strains isolated on Lowenstein Jensen. We performed the recommended protocol of heat inactivation and extraction. Also, Identification was simultaneously done by other techniques as LCD array MycoDirect 1.7, 16S rRNA and WGS for some MOTT isolates as we were hindered by financial issue. Our six MOTT isolates were identified by MALDI TOF MS as two M. fortuitum (33.3%) isolates, one M. abscessus (16.7%) isolate, one M. porcinum (16.7%) isolate, one M. gordonae (16.7%) isolate and one M. bacteremicum (16.7%) isolate. Matching to MALDI TOF MS identification we could perform identification to M. fortuitum by 16 sRNA which was identifiesd as M. neworleansense and it is closely related specied to M. fortuitum groupM.abscessus which was identified by MALDI TOF was also yielded by WGS. Also, LCD array MycoDirect 1.7 results for five strains with the MALDI TOF were concordance but failed to recognize M.bacteremicum |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
M. fortiutum |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
MALDI-TOF MS |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
MOTT |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Maha Ali khalaf , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Ahmed Awad Elbashar , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Sahar Mohamed Khairat , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Amira |
| Reviser |
Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
