MARC details
| 000 -LEADER |
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| fixed length control field |
03465cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
210814s2021 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
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| Holding library |
Deposite |
| 097 ## - Thesis Degree |
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| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| Classification number |
Cai01.07.04.Ph.D.2021.Mo.B |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mosab Abdalla Ahmed Gad |
| 245 10 - TITLE STATEMENT |
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| Title |
Biochemical studies on some plant extracts as anti-cancer / |
| Statement of responsibility, etc. |
Mosab Abdalla Ahmed Gad ; Supervised Fouad Abdelrehim Ahmed , Mohamed Saad Abdellatiif , Hassan Zaki Hassouna |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
دراسات كيميائية حيوية على مستخلصات بعض النباتات كمضاد للسرطان |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Mosab Abdalla Ahmed Gad , |
| Date of publication, distribution, etc. |
2021 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
165 P. : |
| Other physical details |
charts ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Biochemistry |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
The aim of this study was to evaluate the bioactivity of the extracts of the branch of Pinus roxburghii (P. roxburghii) and Nauplius graveolens (N. graveolens) as potential pharmaceutical anticancer drug against human colorectal cancer (HCT-116). The extracts of P. roxburghii and N. graveolens were tested for their antioxidant, antibacterial and antifungal activities. The N. graveolens possessed high significant scavenging activity against DPPH and ABTS assays relative to the moderate antioxidant activity exhibited by P. roxburghii. HPLC separation demonstrated that catechin and chlorogenic acid were predominant polyphenolic compounds in P. roxburghii and N. graveolens, respectively. Moreover, the mentioned extracts exhibited antimicrobial activity against all tested bacteria and fungi. The extracts inhibited the viability of HCT-116 cells with IC₅₀ values of 30.6 og ml-1and 26.5 og m⁻¹, respectively. DNA fragmentation was carried out by gel electrophoresis to detect the apoptotic pathway initiation and the results suggested that the P. roxburghii and N. graveolens extracts induced apoptosis in HCT-116 cells.The apoptotic pathway was tracked through measuring the relative level of mRNA expression of Bcl-2, Bax, Cas3, NF-mB, and PI3k genes by qRT-PCR technique. Using of the IC₅₀ doses of the selected extracts significantly up-regulated Bax, cleaved Cas-3 and down-regulated Bcl-2, NF-{u0138}B and PI3k genes versus GAPDH gene as a house keeping gene compared to control group. The Bax/Bcl-2 ratio was increased up on treatment. The potential toxicity of extracts of P. roxburghii and N. graveolens was assessed.The toxicity profile and the antitumor effect of the extracts of the two plants were tested in vivo in mice model. The results showed that the LD₅₀ of P. roxburghii was 1708.3 mg/ kg body weight and of N. graveolens 1562.5 mg/kg body weight.The results showed that this treatment led to a failure in the liver and kidney function tests. A second trial was designed, in which the mice were administered intragastrically day after day for a whole month with 1/10 the dose of the LD₅₀ of either plant extract |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
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| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Antibacterial and Antifungal activities |
| 653 #4 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Colorectal cancer |
| 653 #4 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
P. roxburghii and N. graveolens extracts |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Fouad Abdelrehim Ahmed , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hassan Zaki Hassouna , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Saad Abdellatiif , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
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| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Shimaa |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
