Studying immune reprogramming associating changes in carbohydrate metabolism upon influenza infection using an in vitro cell cultured immortalized macrophages system / (Record no. 81830)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03237cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032806.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210823s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.01.Ph.D.2021.Ka.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Kareem Mohamed Awad |
| 245 10 - TITLE STATEMENT | |
| Title | Studying immune reprogramming associating changes in carbohydrate metabolism upon influenza infection using an in vitro cell cultured immortalized macrophages system / |
| Statement of responsibility, etc. | Kareem Mohamed Awad ; Supervised Tarek Kamal Motawi , Mahmoud Mohamed Bahgat , Amany Sayed Maghrabi |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة التغيرات المصاحبة لإعادة البرمجة المناعية فى أيض الكربوهيدرات الناجمة عن عدوى الانفلونزا باستخدام نظام معملى مبنى على مزارع الخلايا المناعية الآكلة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Kareem Mohamed Awad , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 102 P. : |
| Other physical details | charts , facimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Alteration of glucose metabolism specifically in immune cells has major health consequences. We monitored changes that took place in glycolytic enzymes, the pyruvate end product of glycolysis, tumor necrosis factor Ü (TNFÜ) and toll-like receptors (TLRs) both at the transcriptional and translational levels upon direct interaction between PR8-H1N1 and the human monocytes U937 in vitro system. Glucose concentration in this system was monitored following stimulation of the cells with pathogen or non-pathogen stimuli.U937 were first treated with H1N1 infectious viral particles or phorbol-12- myristate-13-acetate (PMA) or left untreated and later infected with H1N1 virus.Levels of phosphofructokinase 1 (PFK1) and pyruvate were biochemically quantified. Also, levels of TNFÜ, TLR3 and TLR7 were measured by ELISA. The transcriptional profiles of PFKs, inflammatory cytokines, TLR3 and TLR7 were relatively quantified by qRT-PCR.The results generally revealed significant changes in both the transcriptional and translational profiles of the studied biochemical and immunological parameters upon influenza infection in a time-dependent manner.Results showed also that regardless to whether the initial stimuli on U937 cells were of pathogen or non-pathogen origins, challenge infection by H1N1 causes a significant reduction of glucose levels 36 hours post infection. In conclusion, H1N1 infection has a direct effect on the glucose uptake of U937 cells in vitro and triggers transcriptional and translational changes in immortalized human monocytes which might serve as markers for infection subject for further validation for their specificities. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Glycolysis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Influenza |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Monocytes |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amany Sayed Maghrabi , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud Mohamed Bahgat , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tarek Kamal Motawi , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.01.Ph.D.2021.Ka.S | 01010110084000000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.01.Ph.D.2021.Ka.S | 01020110084000000 | 22.09.2023 | CD - Rom | 84000.CD |