Molecular characterization of virulence genes of chlamydophila psittaci isolated from avian sources and its importance / (Record no. 82127)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03233cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032815.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210914s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.Ph.D.2021.Am.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Amal Mahmoud Abdu Mohamed |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular characterization of virulence genes of chlamydophila psittaci isolated from avian sources and its importance / |
| Statement of responsibility, etc. | Amal Mahmoud Abdu Mohamed ; Supervised Kamelia Mahmoud Osman , Hadia Abdelreheem Ali |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التوصيف الجزيئى لجينات الضراوة المصاحبة لعترات الكلاميدوفيلا سيتاسى المعزولة من الدواجن ومدى أهميتها |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Amal Mahmoud Abdu Mohamed , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 98 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: C. psittacci is one of the most prevalent infections in aviculture and represents the most important animal chlamydiosis of zoonotic character. Purpose: To determine the most proper diagnostic tool for C. psittaci via comparing between different methods applied on this study, in addition to the molecular characterization of chlamydia psittaci isolates.Methodology: 233 serum samples, 330 fecal swabs and 736 internal organs (liver, lung, heart and spleen) were obtained from ducks, ostriches, quail and pigeons.Sixty four tracheal swabs were obtained fron ostrich. C. psittaci was isolated from the samples by Chicken embryo inoculation via yolk sac method. Impression smears were made from the collected yolk sac membranes, stained with Gimenez stain and examined under an oil immersion to detect the presence of chlamydial inclusions. Immunoperoxidase test was used for detection of C. psittaci inclusion bodies in the yolk sac impression smears. Complement fixation test was used for detection of antibodies in the serum samples. Then molecular characterization of chromosomal DNA of C. psittaci was performed by PCR. Results: Positive samples of isolation showed dwarfed and congested embryos with congestion of their yolk sac vessels. Immuno-peroxidase stain positive slides showed discrete, densely labeled brown inclusion bodies was seen with in transparent background. The PCR results of chlamydia psittaci isolates showed that, in fecal samples, ostrich species had positive percentage for chlamydia psittaci (91.7 %), pigeon (86.7 %), quail (85.7 %), duck (80 %). In the tracheal swabs, ostrich species were positive for chlamydia psittaci (83.3 %). Samples of each studied species were positive for the presence of 16S rRNA gene of chlamydia psittaci, and, out of 16 samples, 16 (100%) was positive for ompA |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Chlamydia psittaci |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Molecular diagnosis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Psittacosis |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hadia Abdelreheem Ali , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kamelia Mahmoud Osman , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.Ph.D.2021.Am.M | 01010110084183000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.Ph.D.2021.Am.M | 01020110084183000 | 22.09.2023 | CD - Rom | 84183.CD |