MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03213cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
211012s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.10.17.Ph.D.2021.Wa.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Walaa Mostafa Ahmed |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Investigation of mutations in prevalent NDV genotype VIId in Egypt / |
| Statement of responsibility, etc. |
Walaa Mostafa Ahmed ; Supervised Ahmed A. Elsanousi , Ayman Eldeeb , Walid Kilany |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
استقصاء الطفرات فى فيروس مرض النيوكاسل النوع الوراثى السابع (دى) المنتشر فى مصر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Walaa Mostafa Ahmed , |
| Date of publication, distribution, etc. |
2021 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
78 P. : |
| Other physical details |
charts , fascimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The outbreaks caused by Newcastle disease (ND) continues to spread in Egypt in poultry flocks resulting in high mortalities and serious economic losses. In this study, 450 samples were collected from different chicken farms showing clinical signs from many Egyptian governorates between 2015 and 2018. Out of the 450 samples, 95 (95/450; 21%) were positive for velogenic NDV based on specific real-time RT-PCR. 13 selected samples were subjected to virus isolation and tested for the haemagglutination activity by HI.These samples were subjected to conventional RT-PCR using primers amplify the fusion (F) gene for genetic analysis based on sequencing and phylogeny. Results of the sequencing analysis indicated the belonging of the samples to genotype VII due to the presence of the characteristic amino acid sequences of the F0 protein pro-teolytic cleavage site motifs (112RRQKRF1117). This means the continuous circulation and presence of this genotype in Egypt. Moreover, Phylogeny analyses revealed that the detected NDVs are clustering within velogenic subgenotype VIId with close relationship to previously isolated Egyptian NDVs. For more confirmation, the pathogenicity of the seven selected isolates were evaluated by inoculation of five 10-day old SPF ECE with fresh infective diluted (1/10) allantoic fluid and incubated at 37{u00B0}C. The incubated eggs were examined twice daily to record deaths for determination of the Mean death time (MDT). The results ranged from 54-60 hours which confirms that the isolates are velogenic. In addition to the MDT, Intravenous pathogenicity index (IVPI) was done, 5 6-week-old chickens were inoculated intravenously with the allantoic fluids and observed for 10 days, the chickens was observed every 24. IVPI for samples VRL1, VRL2, VRL3, VRL4, VRL5, VRL6 and VRL8 were 2.26, 1.4, 1.15, 1.94, 1.32, 1.98 and 1.84 respectively. Also, symptoms caused by the NDVs in inoculated Chickens were very characteristic to velogenic pathotypes |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
NDVs |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Newcastle disease |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Real-time PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ahmed A. Elsanousi , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ayman Eldeeb , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Walid Kilany , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Shimaa |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
