BRAF p.V600E gene mutation detection in cancer patients : (Record no. 82602)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02722cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211017s2021 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.07.Ph.D.2021.Ma.B |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | May Mahmoud Aly Kaooh |
| 245 10 - TITLE STATEMENT | |
| Title | BRAF p.V600E gene mutation detection in cancer patients : |
| Remainder of title | Using high resolution melting analysis alone versus combined HRM and COLD-PCR / |
| Statement of responsibility, etc. | May Mahmoud Aly Kaooh ; Supervised Sahar Abdelatty Sharaf , Amal Abdelwahab , Marwa Mahmoud Abdelaziz |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | باستخدام تحليل الذوبان عالى الدقه منفردا مقابله مجتمعا مع تفاعل البلمره المتسلسل : |
| Remainder of title | الكشف عن الطفره الجينيه ب راف (بى .فى 600اى)السرطان |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | May Mahmoud Aly Kaooh , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 93 P . : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Thev-raf murine sarcoma viral oncogene homolog B1 (BRAF) is an oncogene and most frequently mutated in RAF family of kinases. Out of BRAF mutations, BRAF p.V600E is the commonest, which can be linked to cancer development especially in melanoma, colon cancer and hairy cell leukemia. BRAF mutation status has emerged as an important predictive marker for eligibility of BRAF inhibitors with promising outcome. High-resolution melting analysis (HRM) can provide sensitive low cost detection of BRAF mutation which sensitivity can be improved when combined with COLD PCR. Aim of the work:Detection of BRAF p.V600E gene mutation in the studied group of hematological malignancy patients using HRM alone, followed by its detection using combined HRM with COLD PCR to enrich minor alleles and comparing both results. Subjects and methods:Thirty eight samples of hematological malignancies that were scanned for BRAF p.V600E mutation using High resolution melting analysis (HRM) alone and combined HRM and COLD PCR. Results:When HRM was used alone for BRAF p.V600E mutation screening only 3 cases out of 38 were positive for the mutation, while when HRM was combined with COLD PCR 8 cases (21.1%) were positive for the mutationined HRM with COLD PCR compared to HRM alone and with Sanger sequencing |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | BRAF |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | COLD-PCR |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | HRM |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amal Abdelwahab , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marwa Mahmoud Abdelaziz , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sahar Abdelatty Sharaf , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.07.Ph.D.2021.Ma.B | 01010110084434000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.07.Ph.D.2021.Ma.B | 01020110084434000 | 22.09.2023 | CD - Rom | 84434.CD |