In vitro study : (Record no. 82888)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03852cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032838.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211031s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.11.03.Ph.D.2021.Ma.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Manar Mounir Youssef Amin |
| 245 10 - TITLE STATEMENT | |
| Title | In vitro study : |
| Remainder of title | To evaluate the effect of granulocyte colony stimulating factor on colorectal adenocarcinoma and on mesenchymal stem cells transdifferentiation into cancer stem cells by cancer cells derived exosomes / |
| Statement of responsibility, etc. | Manar Mounir Youssef Amin ; Supervised Dina Sabry Abdelfattah , Azza Abusree Ahmed , Abeer Mostafa Ali |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة معملية لتقييم تأثير العامل المحفز لمستعمرة الخلايا المحببة على سرطان القولون والشرج وعلى تحور الخلايا الجذعية التى تعرضت للاكسوسوم المستخلصة من الخلايا السرطانية إلى خلايا |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Manar Mounir Youssef Amin , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 175 P. : |
| Other physical details | charts , fascimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background& Aim:Colorectal cancer (CRC) is one of the most common and lethal malignancies with poor prognosis in the majorityof cases. Cancer stem cells (CSCs) are type of cells within the tumor microenvironment that play a role in cancer initiation, progression, and recurrence.Thus, it is crucial to developnew therapeutic approachfor CRC treatmenteffective against CSCs.Granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor currently used to treat neutropenia in cancer patients. Somestudies reported direct antitumor effect of G-CSF.The aim of our study is to investigate the effect of G-CSF on CRC cells' proliferation,the expression of CRC exosomal micro ribonucleic acid (miRNA), long non coding RNA (LncRNA) responsible for cancer progression, and to evaluate the capability of G-CSF to attenuate the potentiality of CRC exosomes to induce bone marrow derived mesenchymal stem cells (BM-MSCs)transdifferentiation intoCSCs. Materials & methods:CRC cells were cultured and divided into 2 groups. 1st group: untreated cancer cells; 2nd group:CRC cells treated with G-CSF. Cell proliferation of both groups was assessed by methyl thiazol tetrazolium (MTT) assay.Exosomes from both groups were isolatedfor molecular studies [Evaluation of the expression levels of exosomal miRNA-21, miRNA-215, LncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1), programmed cell death 4 (PDCD4), thymidylate synthase (TYMS), dihydrofolate reductase (DHFR), and beta catenin (Ý-catenin) by quantitative real time polymerase chain reaction (qRT PCR)], and then co-cultered with BM-MSCs.The transdifferentiation of BM-MSCs into CSCs under the effect of co-cultured exosomes was assessed by transmission electron microscopy (TEM), flow cytometry, and molecular studies. Results:Molecular studies revealed that:there was a significant decrease in the expression levels of miRNA-21, LncRNA MALAT-1, Ý-catenin, TYMS and DHFR, while there was a significant increase in the expression levels of miRNA-215 and PDCD4 in exosomes derived from CRC cells pre-treated with G-CSF compared to exosomes derived from untreated CRC cells |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Cancer stem cells |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Colorectal cancer |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Exosomes |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abeer Mostafa Ali , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Azza Abusree Ahmed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dina Sabry Abdelfattah , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.11.03.Ph.D.2021.Ma.I | 01010110084593000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.11.03.Ph.D.2021.Ma.I | 01020110084593000 | 22.09.2023 | CD - Rom | 84593.CD |