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Isolation and characterization of bacteria from crude Egyptian petroleum oil reservoirs and their potential use in enhanced oil recovery / (Record no. 82993)

MARC details
000 -LEADER
fixed length control field 03659cam a2200313 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 211106s2021 ua dh f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level M.Sc
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.08.06.M.Sc.2021.Yo.I
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Yomna Ismail Taha Abdelhameed
245 10 - TITLE STATEMENT
Title Isolation and characterization of bacteria from crude Egyptian petroleum oil reservoirs and their potential use in enhanced oil recovery /
Statement of responsibility, etc. Yomna Ismail Taha Abdelhameed ; Supervised Ramy K. Aziz , Noha M. Gamaleldin
246 15 - VARYING FORM OF TITLE
Title proper/short title عزل البكتريا من خزانات البترول الخام المصرى وتحديد خصائصها و استخدامها المحتمل فى تحسين استخراج البترول
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Yomna Ismail Taha Abdelhameed ,
Date of publication, distribution, etc. 2021
300 ## - PHYSICAL DESCRIPTION
Extent 96 P. :
Other physical details charts , facsimiles ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520 ## - SUMMARY, ETC.
Summary, etc. A broad range of bacteria have been detected in or isolated from oil field samples either by culture-based or molecular techniques. Facultatively aerobic, aerobic and other types of microorganisms have been detected in oil samples. Using microorganisms and their byproducts in a technique called 2microbial enhanced oil recovery(MEOR)3 can help recover huge amounts of crude oil from depleted oil reservoirs. In an attempt to study the nature of the bacteria indigenous to the Egyptian oil reservoirs and its characteristics, direct isolation using LB, TSB and MSM was performed on17 different samples of crude oil, formation water and condensate collected from 12 different Egyptian crude oil reservoirs.To this end, 51 bacterial isolates were collected, identified byGram staining, biochemical tests, VITEK 2 and 16S rRNA gene sequencing. The identified isolates belonged to several different genera, but mostly to the genera Bacillus (19 strains) and Pseudomonas (8 strains).Generally, bacteria found in oil reservoirs possess unique metabolic functions that allows them to utilize hydrocarbons and produce biosurfactants and biopolymers among other compounds, which make them the ideal candidate for the development of the MEOR technology. Here we screened the isolates for their ability to produce biosurfactants by different methods: the drop collapse, penetration assay and oil spreading technique. Several isolateswere positive with more than one of the used methods; however, only two of themhad highest scores with all threemethods.These two isolates were identified as Bacillus subtilis and Pseudomonas aeruginosa.In addition to screening the collected isolates for the production of biosurfactants, we also screened them for their ability to produce biopolymer, using YM culture media and fermentation media.Thirty-sevenstrains were found to producebiopolymer, in amounts ranging from 34 to 357.3 mg. The isolates that produced the largest amounts of biopolymer were identified as Bacillus safensis, Bacillus licheniformis and Rhizobium radiobacter. A sample of the extracellular polymeric substance (EPS) collected in this study was chosen for further characterization and identification.This sample was produced by Bacillus licheniformis, pH of the media after fermentation was 7.02 and the weight of the product was 234.3 mg.
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Biosurfactant
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Crude oil
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term MEOR
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Noha M. Gamal Eldin ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Ramy K. Aziz ,
Relator term
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Shimaa
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.08.06.M.Sc.2021.Yo.I 01010110084658000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.08.06.M.Sc.2021.Yo.I 01020110084658000 22.09.2023 CD - Rom 84658.CD