Cryoprotectants and gene expression of cryopreserved oocytes and embryos in buffalo / (Record no. 83680)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02716cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211225s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.14.Ph.D.2021.Es.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Esraa Aly Sayed Ismail |
| 245 10 - TITLE STATEMENT | |
| Title | Cryoprotectants and gene expression of cryopreserved oocytes and embryos in buffalo / |
| Statement of responsibility, etc. | Esraa Aly Sayed Ismail ; Supervised Mohamed A. I. Elsayed , Nabil A. Hemeida , Omaima M. Kandil |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | عوامل الوقاية من التجميد والتعبير الجينى لبويضات وأجنة الجاموس المحفوظة بالتجميد |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Esraa Aly Sayed Ismail , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 120 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This work was carried out for four successive years (2018, 2019 , 2020 and 2021) in order to investigate the effect of different cryoprotectant agents on 1) vitrified/thawed buffalo oocytes developmental competence, 2) viability of vitrified/thawed transferable buffalo embryos and 3) gene expression of in vitro vitrified/thawed matured buffalo oocytes and embryos. Excellent and good oocytes were cultured in TCM-199 medium at 38.5 {u00BA}C, 5% CO2 for 22 hrs, then fertilized using frozen semen and cultured on SOF media. Matured oocytes or transferable embryos were vitrified in DMSO, EG or combination of DMSO+EG by two steps procedure and stored in LN. The morphologically normal vitrified/thawed in vitro matured oocytes were fertilized using frozen semen and cultured on SOF media for detection the cleavage and transferable embryos rates to assess its viability. Fresh matured buffalo oocytes/embryos and recovered vitrified/thawed buffalo oocytes/ embryos were stained with Hochest stain to assess the stage of maturation of in vitro matured oocytes or cell count of in vitro produced embryos and stained with Mitotricker red for detection the mitochondrial distribution and intensity. mRNA was extracted from vitrified/thawed buffalo in vitro matured oocytes or in vitro produced embryos vs fresh matured oocytes or embryos using Picopure kits, cDNA was synthesized by QuantiTect Reverse Transcription kits |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Buffalo |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | In Vitro Embryo Production |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Vitrification |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed A. I. Elsayed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nabil A. Hemeida , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Omaima M. Kandil , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.14.Ph.D.2021.Es.C | 01010110085049000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.14.Ph.D.2021.Es.C | 01020110085049000 | 22.09.2023 | CD - Rom | 85049.CD |