Improving viability and cryotolerance of in vitro produced cattle embryos / (Record no. 83737)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03051cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211227s2021 ua d f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.14.Ph.D.2021.Ya.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Yasser Hussein Ali Saber |
| 245 10 - TITLE STATEMENT | |
| Title | Improving viability and cryotolerance of in vitro produced cattle embryos / |
| Statement of responsibility, etc. | Yasser Hussein Ali Saber ; Supervised Adel A. M. Seida , Refaat S. A. Ragab , Wahid M. M. Ahmed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تحسين حيوية وتحمل التجميد لأجنة الأبقار المنتجة معمليا |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Yasser Hussein Ali Saber , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 118 P. : |
| Other physical details | charts ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In vitro embryo production (IVEP) is considered as one of the most important technologies in cattle industry due to production of superior quality embryos with high developmental competence that use at low costs in embryo transfer programs. So, improvement of in vitro culture systems are the most critical and essential step to keep on this biotechnology. Hence, the current experiments were designed. Experiment 1, aimed to (I) study the effect of phenazine ethosulfate (PES) supplementation in culture media on the selected miRNAs (miR-205, miR-26a-5p, and let-7b) and their target genes (OCT4, DNMT, CASP3, ATF6, ATP5ME, and ELOVL5), during bovine embryo production. Therefore, a group of two-day bovine embryos was cultured in a medium with lipid-reducing agent, PES (0.3 mM). Another group of embryos without PES was left as a control. Embryos were vitrified and morphologically examined after warming and the viability was evaluated by culturing for 24 h. After evaluation, embryos were classified as good or poor. Afterwards, embryos (blastocyst and morula) were kept at -80{u00B0}C for RNA extraction and qRT-PCR of miRNAs and their targets. Results revealed that the rate of morula was higher (P<0.01) in treated compared to control groups. After vitrifications, the percentage of good quality embryos increased in treated than control groups. Additionally, the rate of dead embryos was high in control groups.The Let-7b and miR-205 were significantly over-expressed in the treated good as well as poor embryos compared to control (untreated) good and poor embryos, respectively. However, miR-26 was suppressed in the treated good and poor embryos compared to control (untreated) embryos, respectively. Both of OCT4and DNMT1 transcripts up-regulated in the treated (good& poor) embryos compared to control groups |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Bovine |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Gene expression |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Phenazine ethosulfate |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Adel A. M. Seida , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Refaat S. A. Ragab , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wahid M. M. Ahmed , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shimaa |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.14.Ph.D.2021.Ya.I | 01010110085094000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.14.Ph.D.2021.Ya.I | 01020110085094000 | 22.09.2023 | CD - Rom | 85094.CD |