Effects of curcumin supplementation on viability and Antioxidant capacity of buffalo granulosa cells Under in vitro culture conditions / (Record no. 84249)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03273cam a2200361 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032921.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220217s2021 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.03.M.Sc.2021.Sh.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Sherif Mohamed Abdelhameed Omara |
| 245 10 - TITLE STATEMENT | |
| Title | Effects of curcumin supplementation on viability and Antioxidant capacity of buffalo granulosa cells Under in vitro culture conditions / |
| Statement of responsibility, etc. | Sherif Mohamed Abdelhameed Omara ; Supervised Nasser Ghanem Othman , Marwa Said Faheem , Ayman Moustafa Saeed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تأث{u٠٦أأ}رات إضافة الكركم على ح{u٠٦أأ}و{u٠٦أأ}ة والنشاط المضاد للأكسدة لخلا{u٠٦أأ}ا الجاموس الحب{u٠٦أأ}ب{u٠٦أأ}ة التى تم تنم{u٠٦أأ}تھا معمل{u٠٦أأ}ا |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Sherif Mohamed Abdelhameed Omara , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 92 P . : |
| Other physical details | charts , facsmilies ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Animal Production |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The current study was conducted to investigate the possible protective effects of curcumin supplementation on buffalo granulosa cells (GCs) under in vitro culture conditions. Buffalo ovaries were collected from local abattoir in physiological saline solution at 37oc and transported directly to laboratory. Follicular fluid containing GCs and cumulus-oocyte-complexes were aspirated from antral follicles with diameter 2-8 mm. The collected GCs were seeded (Approximately 375,000 viable cells) in a 8-well culture plate containing 199-tissue culture medium (TCM) and kept at 37{u00B0}C in a humidified atmosphere of 5% CO2. The curcumin was supplemented to TCM media at levels of 1, 2.5, 5 and 10 oM or kept without treatment as control group for 48 hours. The viability of cells was determined using the trypan blue test. Intracellular reactive oxygen species (ROS) level was assessed by measuring the fluorescent intensity of 6-carboxy-2{u2032}, 7{u2032}-dichlorodihydro fluorescein diacetate (H2DCFDA). In addition, mitochondrial activity of GCs was using Mitrocker Red Fluorescent stain. The results of the present study indicated that the viabilityof GCsunder culture conditions were significantly decreased p {u2264} 0.05 in groups treated with 1, 2.5, 5 and 10 oM curcumin (86.0%, 86.26%, 83.0% and 74.0%, respectively) compared to control group (93.60%).The two groups of granulosa cells cultured with 2.5 and 5 oM curcumin recorded greater levels of mitochondrial activity than the groups cultured with 1 oM and 10 oM curcumin. In addition, there was a significant increase in ROS level in group cultured with 10oM curcumin, compared to control and other experimental groups |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Animal production |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Antioxidant |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Buffalo |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Granulosa cells |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ayman Moustafa Saeed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marwa Said Faheem , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nasser Ghanem Othman , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Amira |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.03.M.Sc.2021.Sh.E | 01010110085434000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.03.M.Sc.2021.Sh.E | 01020110085434000 | 22.09.2023 | CD - Rom | 85434.CD |