Study of the immunogenicity of trastuzumab in lab animals and Egyptian patients under its treatment / (Record no. 84378)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04124nam a2200361 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223032925.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220228s2021 ua dhb f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.08.06.M.Sc.2021.Lo.S. |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Lobna Abdelaziz Mohamed Kilany |
| 245 10 - TITLE STATEMENT | |
| Title | Study of the immunogenicity of trastuzumab in lab animals and Egyptian patients under its treatment / |
| Statement of responsibility, etc. | Lobna Abdelaziz Mohamed kilany ; Supervised Hamdallah Hafez Zedan , Mohammad Mabrouk Aboulwafa , Ayman Abdelsameea Gaber |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة التأثيرالمناعى للتراستوزوماب فى حيوانات التجارب وفى المرضى المصريين المستخدم فى علاجهم |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Lobna Abdelaziz Mohamed kilany , |
| Date of publication, distribution, etc. | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 101 P. : |
| Other physical details | charts , facsmiles , maps ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Immunogenicity is a major challenge in drug development and patient care. Clinicians and regulators are familiar with immunogenicity concerns of therapeutics, growth factors, and enzyme replacements. Unlike small molecule therapeutics, the biological therapeutic agents are likely to trigger undesirable immunogenic responses against themselves upon their administration. This imparts a problem that has to be considered upon judging their risk-benefit ratio.The development of monoclonal antibodies (mAbs) counts as one of the major medical steps forward, opening up endless possibilities for research, diagnosis, and treatment of a wide range of various diseases and disorders. Development of both humanized and fully human mAbs was expected to be non-immunogenic, that allow repeated administration without any anti-drug antibodies (ADA) responses. Unfortunately, these expectations were proven to be unrealistic. They fail to completely eliminate mAb immunogenicity and ADA formation; they have reduced the extreme immunogenicity associated with murine origin mAbs, but still, they have shown to induce antibodies that sometimes have an impact on clinical outcomes. Accurate ADA detection is necessary, to provide sufficient information for patient monitoring and clinical intervention. However, assays of ADAs that are developed against mAbs have more challenges as both the analytes and antigens (mAbs) are antibodies. In this study, we tested the immunogenicity developed in patients sera due to the use of trastuzumab and that developed in laboratory animals injected with this recombinant humanized IgG1 monoclonal antibody(trastuzumab). The trastuzumab immunogenicity was assessed in blood samples using an in vitro approach and in laboratory animalsusingin vivo approach. The in vitro approach depended on both detections of anti-trastuzumab antibody (Ab) levels in patients' serum samples withdrawn at different points during the trastuzumab treatment course; and measurement of neutralizing activity of these ADAs using MTT cytotoxicity assay against MCF-7 cell line. The detection of anti-trastuzumab Abs was carried out using the affinity capture elution (ACE) assay methodin both approaches. In vitro analysis of patients' sera for antibodies developed against trastuzumab revealed that this monoclonal antibody has low immunogenicity. Only 1% of samples showed high levels of anti-trastuzumab antibodies which might affect the treatment course. In vivo immunogenicity testing in mice showed also low immunogenicity of trastuzumab that could support the relevant clinical dataapplied in this study |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Microbiology |
| 653 ## - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Immunogenicity |
| 653 ## - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Laboratory animals |
| 653 ## - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Monoclonal Abs |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ayman Abdel Sameea Gaber , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hamdallah Hafez Zedan , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohammad Mabrouk Aboulwafa , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Norhan |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.08.06.M.Sc.2021.Lo.S. | 01010110085488000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.08.06.M.Sc.2021.Lo.S. | 01020110085488000 | 22.09.2023 | CD - Rom | 85488.CD |