MARC details
000 -LEADER |
fixed length control field |
03023nam a2200337 a 4500 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
EG-GiCUC |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
220718s2022 ua d f m 000 0 eng d |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
EG-GiCUC |
Language of cataloging |
eng |
Transcribing agency |
EG-GiCUC |
041 0# - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
049 ## - LOCAL HOLDINGS (OCLC) |
Holding library |
Deposite |
097 ## - Thesis Degree |
Thesis Level |
Ph.D |
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
Classification number |
Cai01.10.10.Ph.D.2022.Mo.S |
100 0# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Mona Mahdy Osman Abdeldaym |
245 10 - TITLE STATEMENT |
Title |
Studies on Virulence and Antimicrobial Susceptibility of Mycoplasma Species Recovered from Sheep and Goat / |
Statement of responsibility, etc. |
Mona Mahdy Osman Abdeldaym ; Supervised Kamelia Mahmoud Osman , Mahmoud Elsaid Hashad , Manal Abuelmakarm Mohamed |
246 15 - VARYING FORM OF TITLE |
Title proper/short title |
دراسات على ضراوة وحساسية الميكوبلازما المعزولة من الاغنام والماعز للمضادات الحيوية |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
Cairo : |
Name of publisher, distributor, etc. |
Mona Mahdy Osman Abdeldaym , |
Date of publication, distribution, etc. |
2022 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
108 P . : |
Other physical details |
charts ; |
Dimensions |
25cm |
502 ## - DISSERTATION NOTE |
Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
520 ## - SUMMARY, ETC. |
Summary, etc. |
In the present study, a total of 400 samples; collected from sheep and goat; were processed for isolation and identification of Mycoplasma species, microbiologically and by molecular techniques. Out of the examined samples, only 43 Mycoplasma isolates (10.75%) were identified as M. ovipneumoniae, M. arginini and untyped Mycoplasma species. M. arginini showed the highest isolation rate from both sheep and goat with incidences of 58.3% and 58.1%, respectively. While, the incidence of M. ovipneumoniae was 11%, and 30% for the untyped Mycoplasma species. Confirmation of the isolates was done by PCR then by sequencing of the PCR products. The sequence results of 5 M. arginini sheep isolates, 4 M. arginini goat isolates, 4 M. ovipneumoniae sheep isolates, one M. ovipneumoniae goat isolate were all submitted to the Genbank taking the accession numbers; MK291433, MK291434, MK291435, MK291436, MK291437, MK640677, MK640679, MK643127, MK774823, MK300052.1, MK 300042.1, MK 361039.1, MK 361029.1 and MK300051.1, respectively, in addition to one untyped Mycoplasma species with the accession number MK910041.The phenotypic virulence traits of sheep and goat isolates including hemolytic activity, biofilm formation, hydrogen sulfide production and catalase enzyme activity were tested. Mycoplasma species isolates showed weak adherence ability to the polystyrene multiwall plates. Phenotypically, the hemolytic activity was recorded in 18 sheep isolates and 13 goat isolates. The antibiotic susceptibility testing by MIC showed sensitivity of both sheep and goat isolates to tulathromycin, tylosin, streptomycin and oxytetracycline. All the isolates were resistant to lincomycin |
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
Additional physical form available note |
Issued also as CD |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Mycoplasma |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Biofilm |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
MIC |
653 ## - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
PCR |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Kamelia Mahmoud Osman , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Mahmoud Elsaid Hashad , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Manal Abuelmakarm Mohamed , |
Relator term |
|
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Amira |
Reviser |
Cataloger |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Enas |
Reviser |
Revisor |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Thesis |