Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

This work was undertaken to study the effect of vanadyl sulfate , an insulin mimetic agent , (vitamin E+selenium) , naturally occurring antioxidants , quercetin , flavonoid possessing an aldose reductase inhibitory effect , separately or in combination , upon some of the brainbnormalities identified in chronically STZ - diabetic ratsTo achieve these goals , we measured brain glucose , ATP , ADP levels as well as ATP / ADP ratioIn addition , we measured brain aldose reductase (AR) , sorbitol dehydrogenase (SDH) and hexokinase (cytosolic (Cyt) and mitochondrial (Mit)) activitiesConjugated diene level was also estimated as an index of the oxidative status of rat brainPlasma glucose level was measured to monitor the hypoglycemic efficiency of the used agentsBody weights were also followed to monitor the physical condition of all animals in the different treated groupsVanadium treatment significantly lowered the elevated plasma glucose level and normalized brain glucose content , ATP / ADP ratio as well as AR , SDH and MitHK activitiesHowever , it failed to reverse the elevated brain ATP , ADP , conjugated diene levels and CytHK activityOn the other hand , (vitE+Se) combined therapy normalized brain glucose , conjugated diene levels , ATP / ADP ratio as well as SDH and HK activities , while , it decreased significantly the elevated blood glucose and AR activityUnfortunally , (vitE+Se) failed to decrease the elevation in brain ATP and ADP levelsOur results showed also that quercetin treatment was effective in normalizing brain glucose , ADP , conjugated diene levels , as well as ATP / ADP ratio and AR activity , and in decreasing the elevated brain ATP level , while , it failed to affect the elevated blood glucose , SDH and HK activitiesCo - administration of (vitE+Se) together with vanadyl sulfate succeeded in decreasing , significantly , the elevated brain conjugated diene level as well as in normalizing the increased CytHK activity shown in vanadyl sulfate - treated group aloneData obtained from (vanadium+quercetin) treated group showed that quercetin could decrease the elevated brain ATP and conjugated diene levels as well as normalize the increased brain ADP level shown in the treated group with vanadium aloneOn the other hand , vanadium succeeded in decreasing the elevated blood glucose level shown in quercetin treated group alone

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