Control of multi-drug resistant escherichia coli infecting the urinary tract in children / Presented by Bassant Alaa Eldin Gomaa ; supervisors Prof. Amira Mohamed AbuTaleb, Dr. Sherif Mohamed Elnagdy.
Material type: TextLanguage: English Summary language: English, Arabic Producer: 2022Description: 116 pages : illustrations ; 25 cm. + CDContent type:- text
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- مقاومه بكتريا الاشرشيا القولونية المقاومة للدواء والتي تصيب المجرى البولي للألطفال [Added title page title]
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Item type | Current library | Home library | Call number | Status | Date due | Barcode | |
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Thesis | قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.12.05.M.Sc.2022.Ba.C (Browse shelf(Opens below)) | Not for loan | 01010110087713000 |
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Thesis (M.Sc.)-Cairo University, 2022.
Bibliography: pages 90-115.
The UTI is one of the most common infections in
children. Out of 850 urine samples, were collected from children suspected to have
urinary tract infection (UTI), 400 samples showed positive cultures. Only 287 (71.75
%) cases out of 400 were diagnosed as the UTI. The UTI was more common among
females 173/287 (60.3 %) than males 114/287 (39.7 %). Children at the age between
3–6 years old were the most susceptible to infection compared to other ages.
Escherichia coli was the main causative pathogen 115/287 (40.06 %) followed by
Klebsiella 50/287 (17.4 %). All isolates of E. coli were resistant to amoxicillin and
ticarcillin. On the other hand, amikacin, meropenem, nitrofurantoin and imipenem
were the most efficient antibiotics and succeeded in controlling high number of E. coli
isolates (88.69 % - 96.52 %). Non ESBL non AmpC producing E. coli isolates were
more susceptible to antibiotics and essential oils than ESBL and AmpC producing E.
coli isolates. Cinnamon oil was the most effective oil against the different strains of E.
coli (ESBL, AmpC and non ESBL non AmpC), followed by thyme, clove, rosemary,
lemon grasses and peppermint. On the other hand, oils of castor, lavender, anise, and
cumin were ineffective against the three strains of E. coli. It was found that MICs
were decreased in cinnamon oil/antibiotic combinations compared to the oil or
antibiotic separately. Fractional inhibitory concentrations indices (FICIs) were
calculated to determine the interaction between cinnamon oil and the selected
antibiotics against the multidrug resistant isolates of E. coli. The synergistic effect
was observed only in cinnamon oil/gentamycin combination in all strains of E. coli
(P-value <0.001). The cnf1 virulence gene was detected by PCR technique. However,
the resistance of the ESBL and AmpC β-lactamase producing E. coli to antibiotics
was statically non-significant in relation to the presence of the cnf1 gene. The lowest
distribution of cnf1 gene was found among non-ESBL non-AmpC producing E. coli
to the antibiotics was significantly related to the presence of the cnf1 gene.
يعد التهاب المسالك البولية أحد أكثر أنواع العدوى شيوعًا في
أطفال. تم جمع 850 عينة بول من أطفال يشتبه في إصابتهم بالمرض
التهاب المسالك البولية (UTI)، أظهرت 400 عينة إيجابية الزرع. فقط 287 (71.75
%) من أصل 400 حالة تم تشخيصها على أنها التهاب المسالك البولية. وكان التهاب المسالك البولية أكثر شيوعا بين
الإناث 173/287 (60.3%) مقابل الذكور 114/287 (39.7%). الأطفال في سن ما بين
وكان الأطفال من 3 إلى 6 سنوات هم الأكثر عرضة للإصابة مقارنة بالأعمار الأخرى.
كانت الإشريكية القولونية هي المسبب الرئيسي للمرض 115/287 (40.06%) يليها
كليبسيلا 50/287 (17.4%). كانت جميع عزلات بكتريا E. coli مقاومة للأموكسيسيلين و
تيكارسيلين. ومن ناحية أخرى، أميكاسين، ميروبينيم، نيتروفورانتوين وإيميبينيم
كانت المضادات الحيوية الأكثر فعالية ونجحت في السيطرة على عدد كبير من بكتيريا الإشريكية القولونية
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