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Purification, characterization, and molecular studies on S- adenosyl methionine synthase in dimorphic fungi / by Gihad Ahmed Sayed Mohammed ; supervisors: by Prof. Dr. Mohsen A. Sayed, Dr. Eman Abdullah M. Ali

By: Contributor(s): Material type: TextTextLanguage: English Summary language: English, Arabic Producer: 2023Description: 112 pages : illustrations ; 25 cm. + CDContent type:
  • text
Media type:
  • Unmediated
Carrier type:
  • volume
Other title:
  • تنقيه, توصيف, و دراسات جزيئيه للإنزيم المصنع للادينوسايل ميثيونين داخل الفطريات ثنائية الشكل [Added title page title]
Subject(s): DDC classification:
  • 580
Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (M.Sc.)-Cairo University, 2023. Summary: Candida spp. was kindly supplied by the micro-analytical center Cairo University. Identification took place biochemically and molecularly. biochemically using the Vitek2 technique, and CHROM-agar media, and molecularly through its DNA extraction, and sequencing. C. albicans was sub-cultured and purified on saboraud–dextrose agar (SDA), yeast agar (YA), and Potatoe dextrose agar (PDA) , while dimorphism phenomenon was enhanced through sub-culturing on RPMI1640 growing media, and then dimorphism was monitored using light microscope, with the estimation of the amount of SAM produced in the yeast cell using HPLC to reflect elevation or depression in the S-adenosyl methionine synthase enzyme. Adomet synthase, extracted and purified from C. albicans, and then activity assay was performed to get well known to the optimal and minimal conditions of the enzyme activity, and the factors that affect its activity. Identifying, and DNA sequencing for the gene that responsible for the enzyme production from C. albicans strain.
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Thesis (M.Sc.)-Cairo University, 2023.

Bibliography: pages 77-112.

Candida spp. was kindly supplied by the micro-analytical center Cairo University. Identification took place biochemically and molecularly. biochemically using the Vitek2 technique, and CHROM-agar media, and molecularly through its DNA extraction, and sequencing.
C. albicans was sub-cultured and purified on saboraud–dextrose agar (SDA), yeast agar (YA), and Potatoe dextrose agar (PDA) , while dimorphism phenomenon was enhanced through sub-culturing on RPMI1640 growing media, and then dimorphism was monitored using light microscope, with the estimation of the amount of SAM produced in the yeast cell using HPLC to reflect elevation or depression in the S-adenosyl methionine synthase enzyme. Adomet synthase, extracted and purified from C. albicans, and then activity assay was performed to get well known to the optimal and minimal conditions of the enzyme activity, and the factors that affect its activity. Identifying, and DNA sequencing for the gene that responsible for the enzyme production from C. albicans strain.

Issued also as CD

Text in English and abstract in Arabic & English.

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