Thesis (M.Sc.)-Cairo University, 2023.

Bibliography: pages 62-88.

Vibrio species are important pathogens that infect fish and marine animals. Twelve types of Vibrio microbes can cause diseases of the human digestive system (gastroenteritis) as a result of consuming infected fish and seafood, such as raw or undercooked oysters, as well as improper handling of infected seafood. The indiscriminate use of antibiotics is one of the main causes and development of microbial resistance to antibiotics, which constitutes a major problem in controlling the outbreak of infection with pathogenic Vibrio species.
In this study, 200 samples of fish and fish products from different sources were tested using standard culture-based methods. 120 samples were positive and confirmed by biochemical tests.


Polymerase Chain Reaction (PCR) was used to confirm the identities of the Vibrio species using the genus-specific and species-specific primers.
The study found that V. parahaemolyticus (44.1%), was the most common species in this research, followed by V. cholerae (38.3%) and V. valnificus (17.5%).
The antimicrobial resistance pattern of pathogenic Vibrio species tested against 15 types of the most commonly used antibiotics which are Ampicillin 30 μg (AM), Oxytetracycline 30 μg (O), Chloramphenicol 30 μg (C) , Nalidixic-acid 30 μg (NA) , Ciprofloxacin 5 μg (CIP) , Cefotaxime 30 μg (CTX) , Norfloxacin 10 μg (NOR) , Trimethoprim 1,25 μg + Sulfamethoxazole 23,75 μg (TS) , Ampicillin 10 μg + Sulbactam 10 μg (SAM) , Streptomycin 10 μg (S) , Cephalothin 30 μg (KF) , Kanamycin 30 μg (K), Erythromycin 15 μg (E) , Tetracycline 30 μg (T) , Gentamicin 200 μg (G).
The isolates showed resistance to more than 6 types of antibiotics with multiple antibiotic resistance (MAR) more than
0.46 to Ampicillin 30 μg, Oxytetracycline 30 μg, Nalidixic acid 30 μg, Streptomycin 10 μg, Cephalothin 30 μg, Erythromycin 15 μg, and Tetracycline 30 μg, indicated that the Vibrio isolates were exposed to high-risk sources of contamination when antibiotics were frequently used.
This MAR results in sever losses in fish aquaculture and the national economy. It has the potential to spread to humans via food chain posing a public health risk.

يعد ميكروب الفيبريو الضارِ من مسببات الأمراض الهامة التي تصيب الأسماك والحيوانات البحرية . يمكن أن يسبب 12 نوعًا من أنواع ميكروب الفيبريو أمراض الجهاز الهضمي للإنسان (التهاب المعدة والأمعاء) نتيجة تناول الأسماك و المأكولات البحرية المصابة، مثل المحار النيئ أو غير المطبوخ جيدًا وكذلك عدم اتباع الإجراءات الصحية اللازمة. يعد الاستخدام العشوائي للمضادات الحيوية من مسببات وتطور مقاومة الميكروب للمضادات الحيوية ، مما يشكل مشكلة كبيرة في السيطرة على تفشي الإصابة بالميكروب الضارِ.

Issues also as CD.

Text in English and abstract in Arabic & English.