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Comparision between nested pcr and quantitative rt - real time pcr in the detection of minimal residual disease in cml patients / Amr Elsayed Ahmed Yousef ; Supervised Mervat Elsayed Mohamed , Sanaa Osman Abdallah , Yasser Ibrahim Hassan Alnahaas

By: Contributor(s): Language: Eng Publication details: Cairo : Amr Elsayed Ahmed Yousef , 2006Description: 123P : charts ; 30cmOther title:
  • مقارنة بين تقنيتى تفاعل انزيم البلمرة المتسلسل المتداخلة و تفاعل انزيم البلمرة المتسلسل فعلية الوقت بأنزيم التحول العكسى فى حساب اقل اثار مرضية فى حالات سرطان الدم النخاعى المزمن [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry Summary: Real - Time RT - PCR has great advantages for estimating transcript levels in a variety of situations These include relative rapid assay times (hours) , reliability and ease of performing replicate analyses In contrast , Nested PCR is a labor procedure requiring a few days to generate useful data We compared the same samples from CML patients by both methods In this study , a total peripheral blood samples from CML patients were analyzed by RT - PCR and Nested PCR , these patients were undergoing therapy by Gleevec Our findings indicated that Nested PCR and Real Time PCR are significantly different
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2006.Am.C. (Browse shelf(Opens below)) Not for loan 01010110046350000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2006.Am.C. (Browse shelf(Opens below)) 46350.CD Not for loan 01020110046350000

Thesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry

Real - Time RT - PCR has great advantages for estimating transcript levels in a variety of situations These include relative rapid assay times (hours) , reliability and ease of performing replicate analyses In contrast , Nested PCR is a labor procedure requiring a few days to generate useful data We compared the same samples from CML patients by both methods In this study , a total peripheral blood samples from CML patients were analyzed by RT - PCR and Nested PCR , these patients were undergoing therapy by Gleevec Our findings indicated that Nested PCR and Real Time PCR are significantly different

Issued also as CD

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