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Molecular characterization of potato leaf roll virus and gene silencing as a strategy for transgenic resistance in plant / Ahmed Kamal Abdelsamad ; Supervised Bahia Yehia Riad , Hamed M. Mazyad , Amr Saad Mohamed

By: Contributor(s): Material type: TextTextLanguage: eng Publication details: Cairo : Ahmed Kamal Abdelsamadb , 2009Description: 120Leaves : charts , facsimiles ; 30cmOther title:
  • الوصف الجزيئى لفيروس التفاف اوراق البطاطس واستخدام طرق التثبيط الجينى كوسيلة مقاومة جينية فى النبات [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry Summary: Potato leafroll disease is caused by Potato virus (PLRV) and transmitted by the aphids (usually Myzus persicae ). The disease causes great yield losses in many countries . In this study the sequence of the coat protein gene (CP ) of PLRV was determined from cDNA clones . The CP gene was cloned into the pBAD- TOPO expression vector upon induction the viral protein was expressed as 6XHis - tagged PLRV fusion protein in Escherichia coli (E. coli ) BL21 cells
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.Ph.D.2009.Ah.M (Browse shelf(Opens below)) Not for loan 01010110053316000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.Ph.D.2009.Ah.M (Browse shelf(Opens below)) 53316.CD Not for loan 01020110053316000

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

Potato leafroll disease is caused by Potato virus (PLRV) and transmitted by the aphids (usually Myzus persicae ). The disease causes great yield losses in many countries . In this study the sequence of the coat protein gene (CP ) of PLRV was determined from cDNA clones . The CP gene was cloned into the pBAD- TOPO expression vector upon induction the viral protein was expressed as 6XHis - tagged PLRV fusion protein in Escherichia coli (E. coli ) BL21 cells

Issued also as CD

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