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Cloning and Expression of Sheep Leptin and Studying ItsBiological Actions / Hanan Abd El Salam Mohamed Ogaly ; Supervised Magdy Ahmed Ghoneim , Mohsen Ahmed Wasfy , Gamal elSayed Essawy

By: Contributor(s): Language: Eng Publication details: Cairo : Hanan Abd El Salam Mohamed Ogaly , 2006Description: 156L : ill ; 30cmOther title:
  • كلونة و انتاج جين الليبتين فى الاغنام [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Biochemistry and Chemistry Of Nutrition Summary: The current study was carried out to isolate the ovine leptin gene of our native sheep , to determine its nucleotide sequence , and to produce recombinant ovine leptin For this purpose the following procedures Samples from different fat depots of male sheep were collected on liquid nitrogen and used for RNA extractionA pair of primer was designed to encode the mature ovine leptin gene with additional cloning sitesThese primers and the extracted RNA together with an optimized condition were used to isolate and amplify ovine leptin cDNA through RT - PCR technique The amplified fragment (insert)
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.04.M.Sc.2006.Ha.C. (Browse shelf(Opens below)) Not for loan 01010110045267000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.04.M.Sc.2006.Ha.C. (Browse shelf(Opens below)) Not for loan 01020110045267000

Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Biochemistry and Chemistry Of Nutrition

The current study was carried out to isolate the ovine leptin gene of our native sheep , to determine its nucleotide sequence , and to produce recombinant ovine leptin For this purpose the following procedures Samples from different fat depots of male sheep were collected on liquid nitrogen and used for RNA extractionA pair of primer was designed to encode the mature ovine leptin gene with additional cloning sitesThese primers and the extracted RNA together with an optimized condition were used to isolate and amplify ovine leptin cDNA through RT - PCR technique The amplified fragment (insert)

Issued also as CD

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