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Construction of Recombinant Baculovirus Expressing HA Protein of Egyptian H5N1 Avian Influenza Virus / Mohammed Abdelmohsen Shahaat Rohaim ; Supervised Hussein Aly Hussein Ahmed , Ismail M. Reda

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mohammed Abdelmohsen Shahaat Rohaim , 2013Description: 317 P. : facsimiles ; 25cmOther title:
  • بناء فيروس باكيلو مدمج يعبر عن بروتين الهيم اجلوتنين لفيروس انفلزنزا الطيور المصرى اتش 5 ان 1 [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology Summary: Egyptian endemic status of Avian Influenza H5N1 accelerating the urgent need to develop potent H5N1 vaccine regimen among other control measures. In the present study, site directed mutagenesis for the full length haemagglutinin (HA) gene of a recent Egyptian strain H5N1 AIV (A/chicken/Egypt/VRLCU/2012) was carried out by deletion of multi- basic cleavage site coding sequence using PCR assays. The mutated HA gene was cloned into the baculovirus transfer plasmid (pFastBac {u2122}) to construct the recombinant pFastBac (rpFastBac-HA). Purified rpFastBac{u2013}HA was transformed into DH10Bac competent cells, transposed with a shuttle vector (Bacmid) to construct the rBacmid-HA which was identi{uFB01}ed by PCR, restriction digestion and sequencing
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2013.Mo.C (Browse shelf(Opens below)) Not for loan 01010110063467000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2013.Mo.C (Browse shelf(Opens below)) 63467.CD Not for loan 01020110063467000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

Egyptian endemic status of Avian Influenza H5N1 accelerating the urgent need to develop potent H5N1 vaccine regimen among other control measures. In the present study, site directed mutagenesis for the full length haemagglutinin (HA) gene of a recent Egyptian strain H5N1 AIV (A/chicken/Egypt/VRLCU/2012) was carried out by deletion of multi- basic cleavage site coding sequence using PCR assays. The mutated HA gene was cloned into the baculovirus transfer plasmid (pFastBac {u2122}) to construct the recombinant pFastBac (rpFastBac-HA). Purified rpFastBac{u2013}HA was transformed into DH10Bac competent cells, transposed with a shuttle vector (Bacmid) to construct the rBacmid-HA which was identi{uFB01}ed by PCR, restriction digestion and sequencing

Issued also as CD

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