Evaluation of some disinfectants against locally isolated Avian Influenza Viruses in Egypt / Abdelrahman Mohammed Gamal ; Supervised Osama Mohamad Kamel Zahran , Sherif Tawfik Moubarak , Hussein Ali Hussien

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Veterinary Hygiene and Management

This study was carried out to evaluate the virucidal ability of some chemical disinfectants commercially available in the Egyptian markets and commonly used in cleaning and disinfecting poultry farms, against Highly Pathogenic Avian Influenza (HPAI) circulating in Egypt and infecting poultry farms since 2006. In this study standard identification and molecular characterization of two isolates of AIV subtype H5N1 isolated from commercial poultry flocks in Egypt during 2011(layer flocks) and 2013(broiler flocks), from different localities in Egypt (isma1lya and bani-sweief respectively) was carried out. Phylogenatic analysis of both strains reveled that the two isolates were of two different clades, the first virus isolated in 2011 belonged to clade 2.2.1.1 a variant virus (A/chicken/Egypt/VRLCU67/ 2011(H5N1)), while the other virus belonged to clade 2.2.1/c a classical virus (A/chicken/Egypt/13VIR3729-4/2013(H5N1)). The tested disinfectants included synthetic phenol compound (PROPHYL 75®), aldhyde and surfactant (ALDEKOL® ), surfactant with different generations of Quats (BIOSENTERY 904®), iodophores (BIOSENTERY IODINE®), Non chlorine oxidizing agent (PERACLEAN 5%®), chlorine releasing agents ( VIRKON S®, Calcium and sodium hypochlorite), beside two clutching and detergent organic Acids (Citric Acid and Formic Acid)

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