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Evaluation of the use of stem cells on a scaffold vehicle in periodontal tissue regeneration / Marwa Mostafa Abdelfattah AbdKhalek ; Supervised Mona Darhous , Shahira Elashiry

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Marwa Mostafa Abdelfattah AbdKhalek , 2014Description: 106 Leaves : charts , facsimiles ; 30cmOther title:
  • تقييم استخدام الخلايا الجذعية على سقالة مركبات لتجديد نسيج اللثة [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Periodontology Summary: Objectives: The aim of the present study was to investigate the effect of proinflammatory cytokine TNFÜ and its decoy TNFR1 on proliferation and osteogenic differentiation of gingival mesenchymal stem/progenitor cells (G-MSCs). Methods: Human G-MSCs were isolated, cultured and magnetically sorted using Stro1 antibodies. Flow cytometric analysis was carried out for the expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146 (Miltenyi-Biotec) and STRO-1 surface markers. Trilineage differentiation and colony-forming units (CFUs) assay were performed. G-MSCs were primed for 72 hours using 1ng/ml TNFÜ (group 1), 1ng/ml TNFÜ and 10ng/ml TNFR1 (group2) and 10ng/ml TNFR1 (group3) while group 4 was unprimed. All four groups were subsequently cultured for two weeks in osteogenic inductive medium. Alzarin red, MTT test and the gene expression of Collagen I, ALP, osteonectin were measured using RT-PCR and normalized to that of GAPDH. Statistical analysis was performed by repeated measures ANOVA (p{u2264}0.05)
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.09.08.Ph.D.2014.Ma.E (Browse shelf(Opens below)) Not for loan 01010110064645000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.09.08.Ph.D.2014.Ma.E (Browse shelf(Opens below)) 64645.CD Not for loan 01020110064645000

Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Periodontology

Objectives: The aim of the present study was to investigate the effect of proinflammatory cytokine TNFÜ and its decoy TNFR1 on proliferation and osteogenic differentiation of gingival mesenchymal stem/progenitor cells (G-MSCs). Methods: Human G-MSCs were isolated, cultured and magnetically sorted using Stro1 antibodies. Flow cytometric analysis was carried out for the expression of CD14, CD34, CD45, CD73, CD90, CD105, CD146 (Miltenyi-Biotec) and STRO-1 surface markers. Trilineage differentiation and colony-forming units (CFUs) assay were performed. G-MSCs were primed for 72 hours using 1ng/ml TNFÜ (group 1), 1ng/ml TNFÜ and 10ng/ml TNFR1 (group2) and 10ng/ml TNFR1 (group3) while group 4 was unprimed. All four groups were subsequently cultured for two weeks in osteogenic inductive medium. Alzarin red, MTT test and the gene expression of Collagen I, ALP, osteonectin were measured using RT-PCR and normalized to that of GAPDH. Statistical analysis was performed by repeated measures ANOVA (p{u2264}0.05)

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