Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

In this study 500 dead or ailing layer were examined clinicaly chickens from differnet farms in Elsharkia, Elkaliubia, Elfayoum, Elgiza, Elmenia, Elbehira and Elmonofia suffering from signs of infectious coryza. The examined chickens showed severe respiratory signs as well as a marked drop in egg production. Postmortem examination revealed conjunctivitis, infra-orbital sinusitis and nasal discharge. Sinus exudate samples were taken and subjected to extensive bacteriological and molecular examination, which included colony morphology, biochemical identification, animal pathogaenicity in embyonated chicken eggs and molecular characterization. The result was isolation and identification of avibacterium paragallinarum using PCR primers specific to the microorganism was used for confirmation of the biochemical results. Serotyping using PCR based protocol resulted in 60% of isolates were page's serovar A and 40% of the isolates were page's serovar C

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