Some epidemiological studies on antigens used in monitoring immune - response of birds vaccinated against avian influenza / Elshaimaa Ismael Sayed Solayman ; Supervised Abdelkarim Mahmoud Abdelkarim , Osama Mohamad Kamel Zahran , Mohga Fouad Mahmoud Badawy
Material type:
- دراسات وبائية حول استخدام الأنتيجينات فى عملية رصد الاستجابة المناعية للطيور للتحصين ضد مرض انفلونزا الطيور [Added title page title]
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قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.10.19.Ph.D.2014.El.S (Browse shelf(Opens below)) | Not for loan | 01010110064764000 | ||
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مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.10.19.Ph.D.2014.El.S (Browse shelf(Opens below)) | 64764.CD | Not for loan | 01020110064764000 |
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Veterinary Hygiene and Management
In this study, we evaluated different factors affecting sero - surveillance against avian influenza in chickens. From November 2010 till December 2013; a total of 382 blood samples for serum and 262 eggs were collected from different chicken commercial farms for haeagglutination inhibition (HI) testing. In case of suspicion of influenza infection; tracheal and cloacal swaps and organ samples were collected for virus isolation and identification. The variant A / chicken / Egypt / VRLCU67 / 2011(H5N1) and the classic A / chicken / Egypt / 13VIR3729 - 4 / 2013 (H5N1) viruses were isolated from a layer and a broiler farms; respectively. Cross - HI and antigenic relatedness testing revealed that the variant strain is distantly related to other tested Egyptian viruses with R% less than 50%. HI testing of sera and egg yolk using the commercial H5N2 Mexican antigen and the isolated viruses revealed the significant difference between results obtained by H5N2 or the classic isolate compared to the variant one. Egg yolk testing for antibodies compared to serum showed high correlation (r = 0.79 - 0.98), putting in mind the type of HI antigen used
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