The mechanism of apoptosis induction by azurin in cancer / Marwa Hasan Soliman Zeid ; Supervised Amr Saad Mohamed , Mervat Elsayed Mohamed , Nadia Mohamed Elguendy.
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TextLanguage: English Publication details: Cairo : Marwa Hasan Soliman Zeid , 2014Description: 106 P. : facsimiles ; 25cmOther title: - آ{uئإؤئ}{uئآئئ}{uئإ٩٤} ا{uئإآ٣}{uئإ٩٨}{uئإء٤}{uئإ٩أ}{uئإ٨إ}ث ا{uئإإ٧}{uئإ٩٨}{uئإء٤}{uئإ٨إ}ر ا{uئإؤئ}{uئإء٨}{uئإئأ}{uئآئإ}{uئإ٨إ} ا{uئإؤئ}{uئإآ٤}{uئإءإ}ط{uئإ٨إ}{uئإإ٧}{uئآئئ}{uئإ٩٤} {uئإ٩١}{uئإ٨إ}{uئإآ٣}{uئإ٩٨}{uئإء٨}{uئإءء}ام ا{uئإئ٧}زور{uئآئإ}{uئإإ٦} [Added title page title]
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قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.12.02.M.Sc.2014.Ma.M (Browse shelf(Opens below)) | Not for loan | 01010110065066000 | ||
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مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.12.02.M.Sc.2014.Ma.M (Browse shelf(Opens below)) | 65066.CD | Not for loan | 01020110065066000 |
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Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry
Azurin is a member of the cupredoxin family of copper containing redox proteins produced by the pathogenic bacteria Pseudonomas Aregunosa. It preferentially penetrates human cancer cells and exerts cytotoxic apoptotic effects or induces cell cycle regression with no apparent activity on normal cells. The purpose of this work is to study the mechanism of apoptosis induction by azurin in breast cancer cells (MCF-7) and colon cancer cells (HCT-116) using Western blotting technique. Azurin was purified using anion exchange and gel filtration chromatography, and its cytotoxicity was assessed by MTT assay. Azurin was found to enhance the intracellular level of the apoptosis inducer p53. In addition, we confirmed that azurin activated intrinsic apoptosis pathway, as reflected by significant activation of caspase- 9 and caspase-7. The involvement of azurin in the extrinsic pathway was confirmed by caspase-8 activation in breast cancer cells (MCF-7)
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