Transformation and expression of glutaredoxin-2 gene into tomato plants / Neama Hussein Osman Abdelati ; Supervised Mona Hashem Ahmed Hussein , Mohamed Hassanien Soliman , Basita Abbas Hussein
Material type: TextLanguage: English Publication details: Cairo : Neama hussein osman abdelati , 2014Description: 131 P. : charts , facsimiles ; 25cmOther title:- نقل و تعبير جين الجلوتاريدوكسين-2 إلى نباتات الطماطم [Added title page title]
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Item type | Current library | Home library | Call number | Copy number | Status | Date due | Barcode | |
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Thesis | قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.07.10.Ph.D.2014.Ne.T (Browse shelf(Opens below)) | Not for loan | 01010110065085000 | |||
CD - Rom | مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.07.10.Ph.D.2014.Ne.T (Browse shelf(Opens below)) | 65085.CD | Not for loan | 01020110065085000 |
Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Genetics
These studies were conducted during the period from 2008 to 2014 at Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt, to improve tomato tolerance to salinity using biotechnological techniques. Development of transgenic tomato plants harboring glutaredoxin- 2 gene (GRX-2) was aimed. In the first experiment, a regeneration system has been established for five tomato cvs, i.e. Flora-Dade, Marmande, Summer Prolific, Castlerock and Super Strain B. Cotyledon and hypocotyl explants from the five tomato cvs were cultured on MS medium supplemented with different concentrations of plant growth regulators. Data showed that, cultivars and BA concentration significantly affected callus induction, shoot induction, and regeneration frequencies for each type of explants. The best cultivar was 2Castlerock3 at 6 mg/l BA. The second experiment was conducted to describe the possibility of producing transgenic tomato plants harbouring the GRX-2 gene, conferring salinity tolerance
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