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Detection of nasopharyngeal carriage of streptococcus pneumoniae in children less than five years Comparison between PCR and culture techniques / Heba Sherif Abdelaziz Fath Allah ; Supervised Amani Ali Elkholy , Magda Badawy , Iman Mohamed Abdelrahman

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Heba Sherif Abdelaziz Fath Allah , 2014Description: 140 P. : charts ; 25cmOther title:
  • الكشف عن حاملي الميكروب العنقودي الرئوي في البلعوم الأنفي للأطفال أقل من سن خمس سنوات : مقارنة بين طريقة المزارع و تفاعل البلمرة المتسلسل [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Background: Streptococcus pneumoniae causes significant morbidity and mortality worldwide. Identification of S. pneumoniae colonization in the nasopharynx is critical for understanding the pathogenesis of pneumococcal diseases and for estimation of vaccine efficacy. Aim of the work: We aimed to detect the rate of nasopharyngeal carriage of S. pneumoniae among children less than 5 years and to use molecular methodology for the detection. Methods: We investigated the colonization characteristics for these pathogens among 200 non- infected children from 6 months to 5 years of age in Cairo, Egypt, in the winter season 2012/2013. Nasopharyngeal specimens were collected using flocked swabs. The specimens were cultures and tested by PCR for detection of Lyt-A and Ply genes. Antimicrobial susceptibility was done using the disk diffusion method and the E test method
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Item type Current library Home library Call number Copy number Status Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2014.He.D (Browse shelf(Opens below)) Not for loan 01010110065114000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2014.He.D (Browse shelf(Opens below)) 65114.CD Not for loan 01020110065114000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Streptococcus pneumoniae causes significant morbidity and mortality worldwide. Identification of S. pneumoniae colonization in the nasopharynx is critical for understanding the pathogenesis of pneumococcal diseases and for estimation of vaccine efficacy. Aim of the work: We aimed to detect the rate of nasopharyngeal carriage of S. pneumoniae among children less than 5 years and to use molecular methodology for the detection. Methods: We investigated the colonization characteristics for these pathogens among 200 non- infected children from 6 months to 5 years of age in Cairo, Egypt, in the winter season 2012/2013. Nasopharyngeal specimens were collected using flocked swabs. The specimens were cultures and tested by PCR for detection of Lyt-A and Ply genes. Antimicrobial susceptibility was done using the disk diffusion method and the E test method

Issued also as CD

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