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Chemical and biological evaluation of type (I) collagen extracted by two techniques from fish scales for dental use / Aiah Abdelwahab Elsayed Elrashidy ; Supervised Azza Abdelzaher Hashem , Shaymaa Ibrahim Habib , Ahmed Yehia Gad

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Aiah Abdelwahab Elsayed Elrashidy , 2014Description: 140 Leaves : charts , facsimiles ; 30cmOther title:
  • تقييم كيميائى و بيولوجي لكولاجين "النوع الأول" المستخرج من قشور الأسماك بواسطة تقنيتين للإستخدام فى طب الأسنان [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Dental Materials Summary: The purpose of this study was to provide an optimum extraction method, purification, characterization and preliminary biological evaluation of type (I) fish scale collagen from fresh water Nile Tilapia (Oreochromis niloticas) fish, which can be effectively used in dental field. Results: Pepsin-solubilized collagen (PSC) was successfully prepared from the Nile Tilapia fish scales. The results of SDS-PAGE showed that extracted collagens were type I collagen. The denaturation temperature was (32 {u00B0}C) .X-Ray Diffraction (XRD) analysis and Fourier-transformed infrared spectra (FTIR) showed that the extracted collagen had a triple helical structure. Active proliferation of BHK-21 cells with no signs of toxicity was evident on collagen gel. Conclusion: 1- Nile Tilapia scales can be used as an effective source for type I collagen extraction. 2- Nile Tilapia collagen fibrillar gel can be used at 37{u00B0}C as a cellular matrix given by its melting temperature (42 {u00B0}C). 3- Collagen gel proved to be biocompatible, and is a promising potential biomaterial for biomedical applications
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.09.10.M.Sc.2014.Ai.C (Browse shelf(Opens below)) Not for loan 01010110065294000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.09.10.M.Sc.2014.Ai.C (Browse shelf(Opens below)) 65294.CD Not for loan 01020110065294000

Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Dental Materials

The purpose of this study was to provide an optimum extraction method, purification, characterization and preliminary biological evaluation of type (I) fish scale collagen from fresh water Nile Tilapia (Oreochromis niloticas) fish, which can be effectively used in dental field. Results: Pepsin-solubilized collagen (PSC) was successfully prepared from the Nile Tilapia fish scales. The results of SDS-PAGE showed that extracted collagens were type I collagen. The denaturation temperature was (32 {u00B0}C) .X-Ray Diffraction (XRD) analysis and Fourier-transformed infrared spectra (FTIR) showed that the extracted collagen had a triple helical structure. Active proliferation of BHK-21 cells with no signs of toxicity was evident on collagen gel. Conclusion: 1- Nile Tilapia scales can be used as an effective source for type I collagen extraction. 2- Nile Tilapia collagen fibrillar gel can be used at 37{u00B0}C as a cellular matrix given by its melting temperature (42 {u00B0}C). 3- Collagen gel proved to be biocompatible, and is a promising potential biomaterial for biomedical applications

Issued also as CD

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