Phenotypic and genotypic characterization of salmonellae isolated from chickens with special reference to antibiotic resistance genes / Mohamed Fathy Abdelfatah ; Supervised Nashwa Abdelsalam Ezzeldeen , Manal Mostafa Zaki , Ahmed Samir Mohamed

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Mohamed Fathy Abdelfatah

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TextLanguage: English Publication details: Cairo : Mohamed Fathy Abdelfatah , 2014Description: 106 P. : facsimiles , photographs ; 25cmOther title:

التوصيف الشكلى و الجينى لمعزولات السالمونيلا من الدجاج مع إشارة خاصة للجينات المقاومة للمضادات الحيوية [Added title page title]

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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: In the present study, 300 samples (liver, spleen, heart and caecum) from freshly dead broilers and 225 cloacal swabs from healthy live layers were collected to investigate the prevalence of salmonella serovars. The isolation of Salmonella from broilers revealed 33 isolates (11%) positive samples and 4 isolates (1.77%) positive samples from layers. Antimicrobial sensitivity testing against 16 antibiotic agents revealed that all of isolates (100%) were sensitive to amikacin, cefepime and imipenem followed by (97.3%) gentamicin, (94.6%) aztreonam and (86.5%) ciprofloxacin. While, (97.3%) of isolates were resistant to nalidixic acid followed by (94.6%) resistant to ampicillin, (83.8%) to tetracycline and (70.3%) to sulphamethoxazole / trimethoprim. Serological survey using ELISA was done on 225 serum samples from the same layer birds to detect carriers. The results revealed that 87 (38.66%) positive samples for salmonella serogroup (B), 93 (41.33%) positive samples for salmonella serogroup (D) and 68 positive samples (30.22 %) for both serogroups (B), (D). Molecular characterization of some ESBLs genes (blaTEM, blaCTX and blaSHV) using PCR showed that 9 isolates were positive for blaTEM gene and 5 were positive for blaCTX. All isolates were negative for blaSHV gene

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Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.M.Sc.2014.Mo.P (Browse shelf(Opens below))		Not for loan	01010110065226000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.M.Sc.2014.Mo.P (Browse shelf(Opens below))	65226.CD	Not for loan	01020110065226000

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Previous	Cai01.10.10.M.Sc.2014.Mo.P Phenotypic and genotypic characterization of pseudomonas aeruginosa and staphylococcus aureus isolated from camel /	Cai01.10.10.M.Sc.2014.Mo.P Phenotypic and genotypic characterization of pseudomonas aeruginosa and staphylococcus aureus isolated from camel /	Cai01.10.10.M.Sc.2014.Mo.P Phenotypic and genotypic characterization of salmonellae isolated from chickens with special reference to antibiotic resistance genes /	Cai01.10.10.M.Sc.2014.Mo.P Phenotypic and genotypic characterization of salmonellae isolated from chickens with special reference to antibiotic resistance genes /	Cai01.10.10.M.Sc.2014.Sa.A Advanced Methods for diagnosis of Salmonella in Poultry /	Cai01.10.10.M.Sc.2014.Sa.A Advanced Methods for diagnosis of Salmonella in Poultry /	Cai01.10.10.M.Sc.2014.Sa.C Comparative studies for serodiagnosis of pasteurella multocida in cattle sera /	Next

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

In the present study, 300 samples (liver, spleen, heart and caecum) from freshly dead broilers and 225 cloacal swabs from healthy live layers were collected to investigate the prevalence of salmonella serovars. The isolation of Salmonella from broilers revealed 33 isolates (11%) positive samples and 4 isolates (1.77%) positive samples from layers. Antimicrobial sensitivity testing against 16 antibiotic agents revealed that all of isolates (100%) were sensitive to amikacin, cefepime and imipenem followed by (97.3%) gentamicin, (94.6%) aztreonam and (86.5%) ciprofloxacin. While, (97.3%) of isolates were resistant to nalidixic acid followed by (94.6%) resistant to ampicillin, (83.8%) to tetracycline and (70.3%) to sulphamethoxazole / trimethoprim. Serological survey using ELISA was done on 225 serum samples from the same layer birds to detect carriers. The results revealed that 87 (38.66%) positive samples for salmonella serogroup (B), 93 (41.33%) positive samples for salmonella serogroup (D) and 68 positive samples (30.22 %) for both serogroups (B), (D). Molecular characterization of some ESBLs genes (blaTEM, blaCTX and blaSHV) using PCR showed that 9 isolates were positive for blaTEM gene and 5 were positive for blaCTX. All isolates were negative for blaSHV gene

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