Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Applications Environmental Metrology Potochemistry and Agriculture

The clinical diagnosis of brucellosis is complicated due to variable incubation periods and absence of apparent clinical signs except abortion. Culture and isolation of brucella species is regarded as the 2gold standard3 test for laboratory diagnosis of brucellosis ,its sensitivity is very low because the Brucella is fastidious micro - organism that can easily be over grown by other contaminating bacteria ,more importantly the procedure is associated with high risk of infections to bacteriology department personnel. Therefore serological tests are often relied upon for the diagnosis of brucellosis. The first component of implementation of an initial screening assay designed for high throughput is to localize the range and frequency of the disease. In case of livestock, the initial screening needs to be inexpensive, easy to perform, rapid, highly sensitive and specific. Several serological tests such as agglutination test, rose bengal test (RBT), card test and other tests have been designed to meet those requirements

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