An assessment of the antibacterial activity, biofilm susceptibility and surface topographic changes of root canal dentin walls irrigated with a newly introduced irrigant Qmix : A comparative in vitro study / Fatma Mohamed Saeed ; Supervised Jealan Mohamed Elshafei , Iman Ezzat Wali , Hany Samy Sadek

Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Endodontics

The aim of this study was to assess and compare the effect of a newly introduced irrigant (Qmix) on enterococcus faecalis biofilm (biofilm susceptibility test), to assess and compare the antibacterial effect of (Qmix) against Enterococcus Faecalis and to study and compare the surfacetopographic changes of root canal dentin walls irrigated with the tested irrigant using scanning electron microscope(SEM). A cellulose nitrate membrane was cut into small discs, placed on the surface of blood agar. A bacterial suspension of E. faecalis was prepared, adjusted to 0.5 Mc farland and 10 microliters were placed on each disc and incubated for 48h. Four discs were used for the first part of this study. One was left untreated and the remaining 3 were treated with the 3 selected test solutions then the membranes were treated with the neutralizer of each irrigant. The untreated and treated membranes were placed on blood agar plates. Then, they were transferred to the central laboratory at the NRC for SEM examination. The remaining 18 discs were transferred to tubes containing the selected irrigants for 2 and 5 minutes. Then the discs were transferred to tubes containing the neutralizing materials of the irrigants then to tubes containing phosphate buffered saline and vortexed. Serial 10-fold dilutions of the suspension were made from which a volume of 20 microliters of each dilution was taken and plated on bile esculin plates. These plates were incubated for 24 hr at 37{u00B0}C

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