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Histological evaluation of bone marrow derived stem cell therapy on experimentally induced osteoarthritis in albino rats{u2019} knee joint / Amal Elham Ahmed Fares ; Supervised Samir Mahmoud Nada , Nagla Mohamed Salama , Mary Atia Morcos

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Amal Elham Ahmed Fares , 2014Description: 152 P. : charts , facsimiles ; 25cmOther title:
  • التقييم الهستولوجى لتأثير العلاج بالخلايا الجذعية النخاعية فى الالتهاب العظمى المفصلى المستحدث تجريبيا فى مفصل الركبة للفئران البيضاء [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Histology Summary: This work aims to evaluate the efficacy of intra-articular injection of bone marrow derived - mesenchymal stem cells (MSCs) in treatment of mono - iodoacetate (MIA) induced osteoarthritis in rat knee joint monitored by histological and immunohistochemical methods. This study was carried out on 45 adult male albino rats. They were classified into 4 Groups: Group I (control group), Group II (osteoarthritic group) in which rats received 1mg of MIA and sacrificed after 2 weeks (in subgroup IIa) and after 4 weeks (in subgroup IIb), group III (stem cell treated group) in which rats received MSCs 2 weeks after MIA injection (in subgroup IIIa) or 4 weeks after MIA (in subgroup IIIb) and sacrificed 2 weeks later and group IV (untreated group) in which rats received PBS 2 weeks after MIA injection (in subgroup IIIa) or 4 weeks after MIA (in subgroup IIIb) and sacrificed 2 weeks later. Sections were taken from rats' knee joints and stained with Hematoxylin and eosin, toluidine blue, immunohistochemichal stains for collagen type II. Sections were examined by light microscopy and immunofluroscent microscopy for PKH26 - labeled MSCs. The mean articular cartilage (AC) thickness, optical density of cartilage matrix proteoglycan and area percent of collagen type II immunoreactivity were measured using image analyzer and statistically analyzed
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.16.Ph.D.2014.Am.I (Browse shelf(Opens below)) Not for loan 01010110065502000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.16.Ph.D.2014.Am.I (Browse shelf(Opens below)) 65502.CD Not for loan 01020110065502000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Histology

This work aims to evaluate the efficacy of intra-articular injection of bone marrow derived - mesenchymal stem cells (MSCs) in treatment of mono - iodoacetate (MIA) induced osteoarthritis in rat knee joint monitored by histological and immunohistochemical methods. This study was carried out on 45 adult male albino rats. They were classified into 4 Groups: Group I (control group), Group II (osteoarthritic group) in which rats received 1mg of MIA and sacrificed after 2 weeks (in subgroup IIa) and after 4 weeks (in subgroup IIb), group III (stem cell treated group) in which rats received MSCs 2 weeks after MIA injection (in subgroup IIIa) or 4 weeks after MIA (in subgroup IIIb) and sacrificed 2 weeks later and group IV (untreated group) in which rats received PBS 2 weeks after MIA injection (in subgroup IIIa) or 4 weeks after MIA (in subgroup IIIb) and sacrificed 2 weeks later. Sections were taken from rats' knee joints and stained with Hematoxylin and eosin, toluidine blue, immunohistochemichal stains for collagen type II. Sections were examined by light microscopy and immunofluroscent microscopy for PKH26 - labeled MSCs. The mean articular cartilage (AC) thickness, optical density of cartilage matrix proteoglycan and area percent of collagen type II immunoreactivity were measured using image analyzer and statistically analyzed

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