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Evaluation of mRNA-based {u200E}approach for the definitive identification of saliva / Reham Nafad Elbendary ; Supervised Hoda Abdelmageed Elghamry , Abla Abdelrahman Ali , Abeer Ahmed Zayed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Reham Nafad Elbendary , 2014Description: 150 P. : charts , facsimiles ; 25cmOther title:
  • تقييم النهج المعتمد على الحمض النووي الريبوزي في الاستعراف النهائي عن سائل اللعاب [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine Toxicology Summary: Background & objectives: The identification of human body fluids or tissues through mRNA-based profiling is very useful for forensic investigations. Saliva used for this study as it is a mirror of the body health and easy sampled by non invasive methods. Aim of this study: Definitive identification of human saliva at different time intervals, using mRNA markers histatin3 (HTN3) & statherin (STATH), by reverse transcriptase PCR (RT PCR).Results: The mean value of the age of the candidates was 29 years old ± 4.06. Males were 55% and females were 45% of all studied groups. There was no statistical significant difference regarding the gender or age variables. Both HTN3 & STATH were highly specific to fresh saliva samples while on the other hand they were not found in fresh blood samples. Mean values of HTN3 and STATH were higher at 1 month and lower at six months in both cotton group and rolling paper groups. There was high significance difference between the HTN3 & STATH in cotton group than in rolling paper group. There was a highly significant strong-negative correlation between levels of genes (HTN3 & STATH) and different time intervals (one, three and six months) in both groups. There was a significance association between males and females regarding the HTN3 values at one month & at three months only
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.13.Ph.D.2014.Re.E (Browse shelf(Opens below)) Not for loan 01010110065399000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.13.Ph.D.2014.Re.E (Browse shelf(Opens below)) 65399.CD Not for loan 01020110065399000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine Toxicology

Background & objectives: The identification of human body fluids or tissues through mRNA-based profiling is very useful for forensic investigations. Saliva used for this study as it is a mirror of the body health and easy sampled by non invasive methods. Aim of this study: Definitive identification of human saliva at different time intervals, using mRNA markers histatin3 (HTN3) & statherin (STATH), by reverse transcriptase PCR (RT PCR).Results: The mean value of the age of the candidates was 29 years old ± 4.06. Males were 55% and females were 45% of all studied groups. There was no statistical significant difference regarding the gender or age variables. Both HTN3 & STATH were highly specific to fresh saliva samples while on the other hand they were not found in fresh blood samples. Mean values of HTN3 and STATH were higher at 1 month and lower at six months in both cotton group and rolling paper groups. There was high significance difference between the HTN3 & STATH in cotton group than in rolling paper group. There was a highly significant strong-negative correlation between levels of genes (HTN3 & STATH) and different time intervals (one, three and six months) in both groups. There was a significance association between males and females regarding the HTN3 values at one month & at three months only

Issued also as CD

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