Thesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Science - Department of laser Application in Medical Biological

Twenty one New Zealand male rabbits weighing 2-2.5 kg and 3 months old were classified into three main groups. The control group did not receive any treatment (n=3 rabbits). The rest of animals (n=18 rabbits), corneal epithelium was injured by 23 syringe needle and blade 15 and was divided into: (A) Normal healing group which was divided into three subgroups (n=3 rabbits each) and the animals were left for normal healing for 1 day,1 week and 4 weeks respectively , (B) Laser treated group which was divided into three subgroups (n=3 rabbits each) and subjected to anterior stromal puncture using Q-switched Nd:YAG laser on corneal sub-epithelium or superficial stroma and the animals were left for 1 day, 1 week and 4 weeks respectively. After the demonstrated period, rabbits were sacrificed, eyes were enucleated and the corneas were isolated for the following measurements: corneal protein content, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) for corneal protein, total antioxidant capacity (TAC), total oxidative capacity (TOC), oxidative stress index (OSI) and histological examination for corneal tissues by light microscope and transmission electron microscope

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