Application of some nanotechnological approaches in rapid detection of feed-borne pathogenic bacteria / Islam Mohamed Tork Gaber Mostafa ; Supervised Mohamed Abdelalim Ali , Islam Ibrahim Omara , Taher Ahmed Salah Eldin

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Islam Mohamed Tork Gaber Mostafa

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TextLanguage: English Publication details: Cairo : Islam Mohamed Tork Gaber Mostafa , 2014Description: 86 P. : charts , facsimiles ; 25cmOther title:

استخدام بعض وسائل النانوتكنولوجى في الكشف السريع عن البكتريا الممرضة في الأعلاف [Added title page title]

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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology Summary: This study was planned to exploit the most recently innovated noble nanoparticles in a highly sensitive colorimetric real-time-assay for detecting E. coli O157:H7 in feedstuffs. Gold nanoparticles (AuNPs) were prepared adopting the citrate method and the obtained colloidal nanoparticle suspension was characterized using the UV{u2013}vis spectroscopy, Dynamic light scattering (DLS), Zeta potential instrument, Transmission Electron Microscopy (TEM) and X{u2019}Pert PRO X-ray Diffraction System (XRD). Results showed that the prepared AuNPs were well dispersed colloidal spherical shaped nanosized particles averaged 40 nm in size having a characteristic surface plasmon resonance band similar to gold nanoparticles and absorbance peak of 530 nm. Zeta potential of the prepared AuNPs verified the negatively charged surfaces of -32 mv. Specific E. coli O157:H7 antiserum was conjugated to the surfaces of the prepared AuNPs and the developed AuNPs-conjugated suspension had a red color. Stability of the AuNPs- E. coli O157:H7 antiserum conjugate suspension was found to be greatly affected by the antibody concentration used in the conjugation mixture.

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Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.07.06.M.Sc.2014.Is.A (Browse shelf(Opens below))		Not for loan		01010110065713000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.07.06.M.Sc.2014.Is.A (Browse shelf(Opens below))	65713.CD	Not for loan		01020110065713000

Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology

This study was planned to exploit the most recently innovated noble nanoparticles in a highly sensitive colorimetric real-time-assay for detecting E. coli O157:H7 in feedstuffs. Gold nanoparticles (AuNPs) were prepared adopting the citrate method and the obtained colloidal nanoparticle suspension was characterized using the UV{u2013}vis spectroscopy, Dynamic light scattering (DLS), Zeta potential instrument, Transmission Electron Microscopy (TEM) and X{u2019}Pert PRO X-ray Diffraction System (XRD). Results showed that the prepared AuNPs were well dispersed colloidal spherical shaped nanosized particles averaged 40 nm in size having a characteristic surface plasmon resonance band similar to gold nanoparticles and absorbance peak of 530 nm. Zeta potential of the prepared AuNPs verified the negatively charged surfaces of -32 mv. Specific E. coli O157:H7 antiserum was conjugated to the surfaces of the prepared AuNPs and the developed AuNPs-conjugated suspension had a red color. Stability of the AuNPs- E. coli O157:H7 antiserum conjugate suspension was found to be greatly affected by the antibody concentration used in the conjugation mixture.

Issued also as CD

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