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Detection of hepcidin M-RNA expression in B-thalassemia patient / Hala Ahmed Abdelsamie ; Supervised Azza Ahmed Aboulenein , Eman Abdelmohsen Shaheen , Mona Mohamed Hamdy Mahmoud

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hala Ahmed Abdelsamie , 2014Description: 111 P. ; 25cmOther title:
  • البحث عن مستوى تعبير الحمض النووي الريبوزى المرسل للهبسيدين في مرضى أنيميا البحر المتوسط [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Background and Objectives: Iron{u2013}Loading anemias are characterized by ineffective erythropoiesis and increased intestinal iron absorption. Erythrocyte transfusions further exacerbate the iron overload. The development of hepcidin- based diagnostics and therapies for iron loading anemias may offer more effective approaches to prevent the toxicity associated with iron overload. The most common iron- loading anemias are major forms of Ý-thalassemia. This study aimed to evaluate the expression of hepcidin mRNA in Ý-thalassemia major patients. Methods: mRNA from a total of 50 Ý-thalassemia major patients and 20 healthy control subjects was extracted, converted to complementary DNA (cDNA) using reverse transcriptase enzyme and then Quantitative detection of hepcidin transcript using ABI Prism (7700) SYBR Green (Real Time-PCR) was done
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2014.Ha.D (Browse shelf(Opens below)) Not for loan 01010110066339000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.M.Sc.2014.Ha.D (Browse shelf(Opens below)) 66339.CD Not for loan 01020110066339000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background and Objectives: Iron{u2013}Loading anemias are characterized by ineffective erythropoiesis and increased intestinal iron absorption. Erythrocyte transfusions further exacerbate the iron overload. The development of hepcidin- based diagnostics and therapies for iron loading anemias may offer more effective approaches to prevent the toxicity associated with iron overload. The most common iron- loading anemias are major forms of Ý-thalassemia. This study aimed to evaluate the expression of hepcidin mRNA in Ý-thalassemia major patients. Methods: mRNA from a total of 50 Ý-thalassemia major patients and 20 healthy control subjects was extracted, converted to complementary DNA (cDNA) using reverse transcriptase enzyme and then Quantitative detection of hepcidin transcript using ABI Prism (7700) SYBR Green (Real Time-PCR) was done

Issued also as CD

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