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Study of different chemical solvents effect on biological activity of natural products / Maysa Mohammed Mohammed Elsaid ; Supervised Sadek Abdou , Mohammed Aly Eldesoky ,Wael Ibrahim Zaki Ashour

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Maysa Mohammed Mohammed Elsaid , 2015Description: 144 P. : facsimiles ; 25cmOther title:
  • دراسة تأثيرالعديد من المذيبات الكيميائية على النشاط الحيوي للمنتجات الطبيعية [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry Summary: Water and ethanol were used as chemical solvents for propolis in this study, and biological activity on bacterial cells(gram negative and gram positive) Hep-2 cell line and splenocytes were studied in addition to quantitative determination of total phenolic and flavonoid by spectrophotometric assay. Results: Antibacterial activity of EEP and WEP studied on (S.aurus, B.subilitus and E .coli). The highest value of inhibition zone for S.aurus in mm was recorded for concentration 100 æg/ ml (14.67±2.08 and 7.00±1.00) for EEP and WEP respectively. The highest value of inhibition zone for B.subilitus in mm was recorded for concentration 100 æg/ ml (15.67±1.53 and 5.33±2.08) for EEP and WEP respectively. The highest value of inhibition zone for E .coli in mm was recorded for concentration 100 æg/ ml (8.33±0.58 and 2.67±1.53) for EEP and WEP respectively, with p value < 0.05. Cytotoxicity of EEP and WEP studied on Hep-2 cell. EEP of concentration in og/ml (0.01, 0.1, 1.0, 10 and 100) reduced the cell viability from 83.05% to 37.83% and WEP with the same concentration reduced the cell viability from 86.44%, to 38.53%. In vitro assays, an inhibitory effect of EEP and WEP on splenocytes proliferation reached maximum effect at a concentration of 100 æg/ml for both EEP (2663.22±145.19 ) and WEP (6520.82±295.19) with (p <0.05)
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2015.Ma.S (Browse shelf(Opens below)) Not for loan 01010110066558000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.02.M.Sc.2015.Ma.S (Browse shelf(Opens below)) 66558.CD Not for loan 01020110066558000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry

Water and ethanol were used as chemical solvents for propolis in this study, and biological activity on bacterial cells(gram negative and gram positive) Hep-2 cell line and splenocytes were studied in addition to quantitative determination of total phenolic and flavonoid by spectrophotometric assay. Results: Antibacterial activity of EEP and WEP studied on (S.aurus, B.subilitus and E .coli). The highest value of inhibition zone for S.aurus in mm was recorded for concentration 100 æg/ ml (14.67±2.08 and 7.00±1.00) for EEP and WEP respectively. The highest value of inhibition zone for B.subilitus in mm was recorded for concentration 100 æg/ ml (15.67±1.53 and 5.33±2.08) for EEP and WEP respectively. The highest value of inhibition zone for E .coli in mm was recorded for concentration 100 æg/ ml (8.33±0.58 and 2.67±1.53) for EEP and WEP respectively, with p value < 0.05. Cytotoxicity of EEP and WEP studied on Hep-2 cell. EEP of concentration in og/ml (0.01, 0.1, 1.0, 10 and 100) reduced the cell viability from 83.05% to 37.83% and WEP with the same concentration reduced the cell viability from 86.44%, to 38.53%. In vitro assays, an inhibitory effect of EEP and WEP on splenocytes proliferation reached maximum effect at a concentration of 100 æg/ml for both EEP (2663.22±145.19 ) and WEP (6520.82±295.19) with (p <0.05)

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