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Diagnosis of bacterial peritonitis by identification of 16s rRNA genes VIA polymerase chain reaction (PCR) / Ahmed Youssef Hassan Ahmed ; Supervised Mona Ahmed Amin , Ahmed Ibrahim Abdelghani Elbadry , Dina Sabry Abdelfattah Hamed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Ahmed Youssef Hassan Ahmed , 2015Description: 122 P. : charts , facsimiles ; 25cmOther title:
  • بواسطة تفاعل البلمرة المتسلسلs16 rRNA تشخيص الالتهاب البكتيرى البريتونى العفوى بالكشف عن جينات [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Internal Medicine Summary: Objectives: evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of SBP. Results: 16s rRNA gene expression in ascitic fluid was positive in all 100 patients suspected to have SBP even in CNNNA, culture negative or patients had PMN < 250 cells/mm3. There was no correlation between 16s rRNA levels and the different laboratory parameters of our cases, it is only correlated with the results of ascitic fluid culture where its levels was higher in culture positive compared to those with culture negative. Conclusion: Identification of bacterial 16s rRNA in ascitic fluid has its significant clinical and diagnostic relevance in suspicion of SBP and its prompt therapy especially in CNNNA. It might be an alternative diagnostic tool to ascitic fluid bacterial culture and PMN in early diagnosis and prompt treatment with subsequent improvement of survival which needs further investigations
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.18.Ph.D.2015.Ah.D (Browse shelf(Opens below)) Not for loan 01010110066691000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.18.Ph.D.2015.Ah.D (Browse shelf(Opens below)) 66691.CD Not for loan 01020110066691000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Internal Medicine

Objectives: evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of SBP. Results: 16s rRNA gene expression in ascitic fluid was positive in all 100 patients suspected to have SBP even in CNNNA, culture negative or patients had PMN < 250 cells/mm3. There was no correlation between 16s rRNA levels and the different laboratory parameters of our cases, it is only correlated with the results of ascitic fluid culture where its levels was higher in culture positive compared to those with culture negative. Conclusion: Identification of bacterial 16s rRNA in ascitic fluid has its significant clinical and diagnostic relevance in suspicion of SBP and its prompt therapy especially in CNNNA. It might be an alternative diagnostic tool to ascitic fluid bacterial culture and PMN in early diagnosis and prompt treatment with subsequent improvement of survival which needs further investigations

Issued also as CD

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