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Immunological and environmental studies on giardia duodenalis in Egypt / Mona Bashir Abdellatif Khalil ; Supervised Azza Mohamed Elamir , Bayumy Bayumy Mostafa , Alyaa Ahmed Farid

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mona Bashir Abdellatif Khalil , 2015Description: 98 P. : charts ; 25cmOther title:
  • درا{uئإآ٣}{uئإ٨إ}ت {uئإإ٣}{uئإإ٨}{uئإ٨إ}{uئإأآ}{uئآئئ}{uئإ٩٤} و {uئإ٩١}{uئآئئ}{uئإ٨أ}{uئآئئ}{uئإ٩٤} {uئإؤئ}{uئإء٠}{uئآئئ}{uئإ٨إ}رد{uئآئإ}{uئإ٨إ}ت ا{uئإئ٩}{uئإ٩آ}{uئإإ٨}{uئإئ٠} {uئإأآ}{uئإآ٨}{uئإءإ} {uئإؤ٣}{uئإئ٠} {uئإإ٣}{uئإآأ}ر [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology Summary: Giardia duodenalis (G. duodenalis) is one of the leading protozoan causes of gastrointestinal illness worldwide. Giardiasis constitutes an important public health problem in Egyptian children. Contamination of the Nile river with fecal materials including viruses and pathogenic protozoa still represents an environmental health hazard in Egypt; especially in rural areas. This study aimed to detection of crude giardia antigen (CGA) by enzyme-linked immunoassaying (ELISA) technique in stool and serum samples of G. duodenalis infected patients and in water samples from areas in which the G. duodenalis infected patients are residence and compare the results with that of the parasitological methods and serodiagnosis in correlation to some environmental factors. According to coproantigens detection by sandwich ELISA, higher sensitivity (96.39%) than parasitological examination (88.75%) was detected. Also, sera samples were employed in sandwich ELISA assay, to detect circulating giardia antigens; the sensitivity of the test was 94.12% which is higher than that of IgM (87.50%) and IgG (86.25%) secretion measurement. Water samples were collected and the giardia concentration was performed using membrane filtration and percoll - sucrose flotation. From ordinary microscopic identification of protozoa stages in water samples, giardia cysts were found in 29 water samples
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2015.Mo.I (Browse shelf(Opens below)) Not for loan 01010110067490000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.21.M.Sc.2015.Mo.I (Browse shelf(Opens below)) 67490.CD Not for loan 01020110067490000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

Giardia duodenalis (G. duodenalis) is one of the leading protozoan causes of gastrointestinal illness worldwide. Giardiasis constitutes an important public health problem in Egyptian children. Contamination of the Nile river with fecal materials including viruses and pathogenic protozoa still represents an environmental health hazard in Egypt; especially in rural areas. This study aimed to detection of crude giardia antigen (CGA) by enzyme-linked immunoassaying (ELISA) technique in stool and serum samples of G. duodenalis infected patients and in water samples from areas in which the G. duodenalis infected patients are residence and compare the results with that of the parasitological methods and serodiagnosis in correlation to some environmental factors. According to coproantigens detection by sandwich ELISA, higher sensitivity (96.39%) than parasitological examination (88.75%) was detected. Also, sera samples were employed in sandwich ELISA assay, to detect circulating giardia antigens; the sensitivity of the test was 94.12% which is higher than that of IgM (87.50%) and IgG (86.25%) secretion measurement. Water samples were collected and the giardia concentration was performed using membrane filtration and percoll - sucrose flotation. From ordinary microscopic identification of protozoa stages in water samples, giardia cysts were found in 29 water samples

Issued also as CD

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