Preparation of elisa kit for detection of antibodies to Lumpy Skin Disease Virus (LSDV) / Nadia Maher Hanna Danial ; Supervised Hussein Aly Hussein Ahmed , Mohamed Samy Saber , Attia Mohamed Samy

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Nadia Maher Hanna Danial

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TextLanguage: English Publication details: Cairo : Nadia Maher Hanna Danial , 2015Description: 166 P. ; 25cmOther title:

تحضير طقم إليزا للكشف عن الأجسام المناعية لفيروس مرض الجلد العقدى [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology Summary: In this study, an ELISA Kit was developed for Detection of antibodies to Lumpy Skin Diseasevirus (LSD).Trial of isolation and characterization of LSD virus from skin nodules samples obtained from EL Gharbia Governorate in year 2009 in Egypt. Skin nodules were propagated in SPF fertile chicken eggs and in MDBK cell line. Titration of suspected LSDV was performed. Characterization of suspected LSDV was carried out using TEM, IFAT and PCR. Screening of 254 serum samples from vaccinated cattle and 14 serum samples from infected cattle obtained from different governorates in Egypt using SNT .92 field serum samples of vaccinated cattlewhich tested by SNT were collected to test using prepared ELISA plate for detection and quantitative of antibodies against Lumpy Skin Disease Virus (LSDV). The evaluation was achieved to evaluate this assay by comparison with Serum Neutralization test (SNT)

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Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.17.Ph.D.2015.Na.P (Browse shelf(Opens below))		Not for loan		01010110067797000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.17.Ph.D.2015.Na.P (Browse shelf(Opens below))	67797.CD	Not for loan		01020110067797000

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

In this study, an ELISA Kit was developed for Detection of antibodies to Lumpy Skin Diseasevirus (LSD).Trial of isolation and characterization of LSD virus from skin nodules samples obtained from EL Gharbia Governorate in year 2009 in Egypt. Skin nodules were propagated in SPF fertile chicken eggs and in MDBK cell line. Titration of suspected LSDV was performed. Characterization of suspected LSDV was carried out using TEM, IFAT and PCR. Screening of 254 serum samples from vaccinated cattle and 14 serum samples from infected cattle obtained from different governorates in Egypt using SNT .92 field serum samples of vaccinated cattlewhich tested by SNT were collected to test using prepared ELISA plate for detection and quantitative of antibodies against Lumpy Skin Disease Virus (LSDV). The evaluation was achieved to evaluate this assay by comparison with Serum Neutralization test (SNT)

Issued also as CD

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