Seminal MicroRNA in infertile men with Varicocele / Mohamed Farag Azmy ; Supervised Taymour Mostafa Ibrahim , Laila Ahmed Rashed , Nashaat Nabil Ismail

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Andrology and S.T.Ds

Materials and Methods: This study included 79 men that were consecutively recruited from the Andrology Department, Kasr El-Aini Hospital, after institutional review board (IRB) approval and informed consent. They were divided into; fertile men without Vx (n=19), fertile men with Vx (n=14), oligoasthenotertatozoospermic (OAT) men without Vx (n= 23) and OAT men with Vx (n= 23). They were subjected to history taking, clinical examination and semen analysis. In their seminal plasma,BAX and BCl2, malondialdehyde (MDA) and glutathione peroxidase (GPx) were estimated in addition to seminal plasma miRNA 122,181a, 34c-5 by quantitative real time {u2013}PCR. Results: Seminal miRNA 122, 181a, 34c-5 demonstrated significant positive correlation with sperm count, sperm motility, sperm normal forms, seminal GPx, seminal BCl2, and with each other and significant negative correlation with seminal BAX, MDA.Seminal miRNAs 122, 181a, 34c-5 demonstrated nonsignificant correlation with Vx grade.There was a signi{uFB01}cant increase in seminal Bax and significant decrease in seminal Bcl2 in infertile men compared with fertile men being exaggerated in infertile men with Vx. There was a signi{uFB01}cant increase in seminal MDA and significant decrease in seminal GPx in infertile men compared with fertile men being exaggerated in infertile men with Vx. There was a signi{uFB01}cant decrease in mean levels of seminal miRNAs in infertile men compared with fertile men being the least in infertile men with Vx

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