Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Histology

The present work aimed at investigating the possible therapeutic effect of garlic extract (GE) on induced wound in non-diabetic and diabetic male albino rat. The possible role of endogenous stem cells (SCs) was also determined. Thirty two adult male albino rats were divided into, Group I (control group) of 8 rats, Group II (Non diabetic wounded group) and Group III (Non diabetic wounded and GE treated group), 6 rats each were exposed to wound induction, in Group III 0.5 ml of 1% GE was locally applied on the wound and on the surrounding skin margins for 6 days/week at an interval of 24 h following wound induction. Group IV (diabetic wounded group) and Group V (diabetic wounded and GE treated group), 6 rats each that received single IP injection of STZ 50 mg / kg dissolved in 0.5 ml citrate buffer then exposed to wound induction and in Group V 0.5 ml of 1% GE was locally applied. Each group was subdivided into subgroups a and b, in each 3 animals were sacrificed 2 and 3weeks respectively. Skin sections including the wound and surrounding skin margins were subjected to histological, immunohistochemical, morphometric and statistical studies: In subgroup IIa, separation of the wound edges, epidermal thickening, diffuse eosinophilic homogenous material extending, detached fibers of the horny layer, impacted keratin between the epidermal layers and nuclear margination in multiple vacuolated epidermal cells were noticed

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