Thesis (Ph.D.) - Cairo University - Institute of African Research and Studies - Department of Natural Resources

This study was performed at the Natural Resources Department, Institute of African Research and Studies, Cairo University, Giza, Egypt. The experiments were done in Horticulture Research Institute, Agriculture Research Center, Fruits Breeding Department - Giza, Egypt during the period 2012 to 2014. The aim of this work was to optimize the culturing conditions for the micropropagation of J. curcas L. by using single node and leaf explants and to study the salinity tolerance by using mutation through tissue culture technique. The highest percentage of survival explants were achieved by using NaOCl at 20%. The highest percentage of survival explants were achieved by using 0.1 g/l MC for 15 minutes. The single node explants cultured on medium supplemented with 2,4-D at 4.0 mg/l recorded the highest callus formation percentage 3.0%. The callus formation percentage range between 0.3 {u2013} 1.7% according to different concentrations of 2,4-D from leaves. By using NAA at 4.0 mg/l gave high percentage of callus formation 2.7%; while, NAA at 1.0 mg/l showed low percentage of callus formation of single nodes. By using NAA at 2.0 and 4.0 mg/l resulted in high percentage of callus formation of leaves. The highest percentage of callus formation 4.0% was recorded with 2,4-D at 1.0 mg/l among NAA, IBA and IAA at 1.0 mg/l of single nodes. The highest percentage of callus formation 3.7, 3.2, 3.8 respectively was recorded with 2,4-D at 1.0 mg/l among different concentration of NAA, IBA and IAA of leaves. BA at 3.0 mg/l with IAA at 3.0 mg/l increased the shoot formation percentage from single node and leaf explants. Using kin at 2.0 mg/l + IBA at 0.2 mg/l was recorded the highest shoot formation percentage from single node and leaf explants. The control treatment was recorded no results

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