Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

Background: MicroRNAs (miRNAs) are small non coding RNAs with suggested association in several conditions. However, their roles in diabetes and coronary artery disease (CAD) complication of diabetes have not been fully studied. Aim: to study the interplay between two miRNAs; 9 and 370, and their correlation with biochemical parameters in type 2 diabetic (T2D) and CAD. Subjects and methods: levels of both miRNAs (using real time PCR) and routine parameters were measured in, patients with T2D, patients with CAD, patients with T2D and CAD, and healthy control subjects. Blood samples were taken from both genders, in the Outpatient clinic in Al Qasr El-Einy teaching hospitals. 200 subjects were divided over four groups, 50 in each group, age matched, diabetes duration was from 10 to 15 years, and not suffering from diseases other than T2D or CAD. Main outcome: miRNA 9 expected to be over expressed in diabetic patients, while miRNA 370 expected to be over expressed in those suffering from CAD. Results: miRNA 9 level was significantly higher in T2D patients and T2D patients with CAD, (1.18±0.07, and 1.31±0.08 respectively), miRNA 370 level was significantly higher in T2D patients, CAD patients, and T2D patients with CAD (0.59±0.05, 1.00±0.05, and 1.20±0.06 respectively), compared to control group at p=0.000. In addition both miRNAs were still significantly associated with each other even after conducting multiple regression analysis. Conclusion: This study associates the possible role of miRNAs in the diagnosis/prognosis of CAD complication of T2D

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