Refining laboratory diagnosis of chronic lymphoid malignancies in leukemic phase / Randa Amin Osman ; Supervised Azza Mahmoud Kamel , Nahla Mohamed Elsharkawy , Essam Hamed Elnoshokaty

Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Clinical Pathology

Neoplasms of mature lymphoid cells include the chronic lymphocytic leukemia and non-Hodgkin lymphomas. This group of diseases is recognized by an immunophenotype that is similar to normal mature lymphoid cells (eg, surface immunoglobulin on mature B cells) and lack of antigenic features of immaturity, such as expression of TdT, CD34, or weak intensity staining for CD45. According to the 4th edition of the WHO classification, the diagnosis of major mature B- cell malignancies is usually based on a combination of morphology, immunophenotype and recurrent cytogenetic aberration. Some other B cell lymphomas still remain challenging for flow cytometrists, particularly where there is no single disease-specific phenotype which characterizes the entity, and there is great heterogeneity between cases. The purpose of this study was to categorize the unclassifiable mature B- cell lymphoid neoplasms using a broad panel of monoclonal antibodies against B- cell antigens and adhesion molecules, in addition to other characteristic molecular genetic abnormalities in a trial to develop an algorithm for testing those unclassifiable cases to reach a definite diagnosis (whenever possible) with the least laboratory workup

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