Role of glycophorin A and endothelial microparticles in Egyptian patients with thalassemia / Mohamed Elshahat AboEltana Eldahma ; Supervised Nohair Soliman , Ilham Youssry , Amal Soliman Nasr

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

MPs display surface proteins of multiple originating cells, including platelets, RBCs, endothelial cells, monocytes, and granulocytes. The aim of this study was to evaluate the role of erythrocyte MPs and glycophorin A in the thrombo embolic risk observed in thalassemic patients and to underline a possible difference between thalassemia major and thalassemia intermedia. Levels of MPs in the peripheral blood were measured by using flow cytometric technique. MPs are identified by their size and the use of monoclonal antibodies (mAb) to determine the cellular origin of the MPs. This study showed that the levels of procoagulant MPs of RBC and endothelial origins are high in patients with Ý- thalassemia intermedia, but their possible deleterious role in endothelial dysfunction remains to be further investigated. Our work showed that 58.33% of TI patients experienced TEE and 41.66% did not, whereas among TM patients, 33% had TEE compared to 67% did not. where 78% of thalassemic patients, who have experienced TEE, were splenectomised in comparison to 22% who were not splenectomised. The association of elevated MP levels with many different pathological states makes them of particular interest for clinical research, and suggests that these tiny vesicles have great potential for the development of new diagnostic assays aimed at identifying early stages of pathological disorders and response to therapy

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