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Role of micro RNA as marker of liver injury in paracetamol acute toxicity / Mai Moustafa Magdi farrage ; Supervised Hala Saied Zaghloul , Abeer Ahmed Zayed , Alaa Mohammed Shehab

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mai Moustafa Magdi farrage , 2015Description: 110 P. : charts , facsimiles ; 25cmOther title:
  • دور الحمض النووي الريبي الدقيق كدلالة علي إصابة الكبد في التسمم الحاد بالباراسيتامول [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine and Toxicology Summary: Background & objectives: Paracetamol (acetaminophen) overdose is one of the most common causes of acute liver injury in the world. Early detection of drug induced liver injury remains a problem as the classic markers for liver injury lack clinical sensitivity and specificity. MiRNA 122 & miRNA 192 are promising biomarkers with high sensitivity and specificity for detection of drug induced liver injury. Aim of this study: The aim of the current study is to investigate the potential utility of miRNAs 122 & 192 as markers of human paracetamol-induced liver injury and to compare miRNA with existing biomarkers of liver injury in humans. Results: The age of all participants ranges between 11-57 years with mean age in group (1) 24.83, in group (2) was 23.2 and in group (3) it was 31.07. We found that 100% of cases were suicidal. Males were 21.4% and females were 78.6% of all studied cases. The mean and standard deviation of paracetamol level (mg/l) in group (1) was 287.34±79.74 and in group (2) it was 31.71±12.67. The mean and standard deviation of liver enzymes (AST & ALT) (IU/L) in the study group (1) was in normal range (31.31±2.71 & 20.57±1.96 respectively). The mean and standard deviation of liver enzymes (AST & ALT) in the study group (2) was in range higher than normal limits (91.80±26.93 & 39.29±13.77respectively). Results of paracetamol level, miRNA 122 levels and miRNA 192 levels showed statistical significance between the study group G3 and both groups of cases (groups G1 & G2). Conclusion: Both types of miRNAs included in the study (122 & 192), are highly sensitive markers of paracetamol induced liver injury in cases both with early and late presentation. Keywords: paracetamol, miRNA, RT-PCR, Drug induced liver injury
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.13.Ph.D.2015.Ma.R (Browse shelf(Opens below)) Not for loan 01010110069004000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.13.Ph.D.2015.Ma.R (Browse shelf(Opens below)) 69004.CD Not for loan 01020110069004000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine and Toxicology

Background & objectives: Paracetamol (acetaminophen) overdose is one of the most common causes of acute liver injury in the world. Early detection of drug induced liver injury remains a problem as the classic markers for liver injury lack clinical sensitivity and specificity. MiRNA 122 & miRNA 192 are promising biomarkers with high sensitivity and specificity for detection of drug induced liver injury. Aim of this study: The aim of the current study is to investigate the potential utility of miRNAs 122 & 192 as markers of human paracetamol-induced liver injury and to compare miRNA with existing biomarkers of liver injury in humans. Results: The age of all participants ranges between 11-57 years with mean age in group (1) 24.83, in group (2) was 23.2 and in group (3) it was 31.07. We found that 100% of cases were suicidal. Males were 21.4% and females were 78.6% of all studied cases. The mean and standard deviation of paracetamol level (mg/l) in group (1) was 287.34±79.74 and in group (2) it was 31.71±12.67. The mean and standard deviation of liver enzymes (AST & ALT) (IU/L) in the study group (1) was in normal range (31.31±2.71 & 20.57±1.96 respectively). The mean and standard deviation of liver enzymes (AST & ALT) in the study group (2) was in range higher than normal limits (91.80±26.93 & 39.29±13.77respectively). Results of paracetamol level, miRNA 122 levels and miRNA 192 levels showed statistical significance between the study group G3 and both groups of cases (groups G1 & G2). Conclusion: Both types of miRNAs included in the study (122 & 192), are highly sensitive markers of paracetamol induced liver injury in cases both with early and late presentation. Keywords: paracetamol, miRNA, RT-PCR, Drug induced liver injury

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