Evaluation of ELISA and gamma interferon techniques for diagnosis of tuberculosis in cattle / Sayed Muhammed Sayed Hassan ; Supervised Mahmoud Essam Hatem Ahmed , Ahmed Samir Mohammad , Emad Mokhtar Riad

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

In the present study, two groups (I and II) of animals were tested. Group I composed of 170 tuberculin reactor cattle and out of them 88 animals (51.8%) were positive to bTB by microscopic examination of direct smears while by culture on L - J medium (4% pyruvate) M.bovis was isolated from 114 animals (67.1%). The bacteriological examination of tissue samples collected from different organs showing tuberculous lesions from group I (170 lymph node, 40 lung, 34 liver and 20 spleen) revealed that by microscopic examination spleen showed the highest percentage of positive results, 55% (11/20), followed by lung 52.5% (21/40) then lymph nodes 51.8% (88/170) and finally liver at a percentage of 50% (17/34), also by culture method spleen had the highest percent of positive results, 70% (14/20), followed by lung, lymph node and liver in a percentage of 67.5% (27/40), 67.1% (114/170) and 61.8% (21 / 34) respectively. On the other hand, group II composed of 200 animals which were subjected to SICTT on two phases, out of them 26 animals were positive reactors, 14 from the first phase and 12 from the second one, of them 19 (73.1%) animals were positive to bTB by culture on L-J (4% pyruvate). Serum samples were collected from group II, before being tested by SICTT, to be tested by ELISA and results revealed that out of the total 200 animals, 20 (10%) animals were positive to bTB when using PPD-B as coating antigen compared to 23 (11.5%) positive animals by using a commercial mixture antige

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