Biochemical and neurological studies of some pesticides on neonatal rats / Khairy Abdelmoneim Ibrahim ; Supervised Amr Saad Mohamed Elsayed , Mohamed Ali Eldesouky , Hala Mohamed Abou Yousef

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

Background/Aims: Early pesticide exposures pose a particular concern since many are neurotoxic to humans and suspected to implicate in neurological diseases. In the present work, independent and combined intoxication with imidacloprid, a neonicotinoid, and esfenvalerate, a pyrethroid, were studied to evaluate their possible adverse effects on developing rats. Methods: Imidacloprid (0.529, 1.058, and 2.116 mg/kg) and esfenvalerate (0.142, 0.284 and 0.568 mg/kg) in addition their mixture at low doses were orally administrated to twenty one days old albino rats (five animals per each one) for consecutive seven days. At the end of the experiment, brain and liver were quickly frozen and saved until being used for ATPases, neurotransmitters and xenobiotic metabolizing enzymes determinations. Also caspase-3, comet assay and DNA fragmentation in brain and liver beside the plasma thyroid hormones were estimated. Results: It was found that brain and liver acetylcholinesterase, monoamine oxidase, paraoxonase and glutathione S-transferase beside brain Mg+2 ATPases were significantly decreased after independent and combined intoxication with two tested pesticides. The same pattern was scored in liver and brain carboxylestrase, total and Na & K ATPases after individual treatments with esfenvalerate at medium and high doses beside with mixture. Plasma T3 and T4 levels also were significantly reduced with esfenvalerate at medium and high doses beside with a mixture in addition plasma T3 were decreased after imidacloprid intoxication at medium and high doses. In contrast caspase-3 and DNA fragmentation were increased in brain and liver after independent and combined intoxication

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